N-Glycosylation patterns of hemolymph glycoproteins from Biomphalaria glabrata strains expressing different susceptibility to Schistosoma mansoni infection

被引：22

作者：

Lehr, Tobias ^{[1
]}

Frank, Sandra ^{[1
]}

Natsuka, Shunji ^{[2
]}

Geyer, Hildegard ^{[1
]}

Beuerlein, Knut ^{[3
]}

Doenhoff, Michael J. ^{[4
]}

Hase, Sumihiro ^{[2
]}

Geyer, Rudolf ^{[1
]}

机构：

[1] Univ Giessen, Fac Med, Inst Biochem, D-35392 Giessen, Germany

[2] Osaka Univ, Grad Sch Sci, Dept Chem, Osaka 5600043, Japan

[3] Univ Giessen, Fac Med, Rudolf Buchheim Inst Pharmakol, D-35392 Giessen, Germany

[4] Univ Nottingham, Sch Biol, Nottingham NG7 2RD, England

来源：

EXPERIMENTAL PARASITOLOGY | 2010年 / 126卷 / 04期

关键词：

Trematode; Schistosoma mansoni infection; Susceptible snails; Resistant snails; Molecular mimicry; Glycoprotein; Carbohydrate epitope; Oligosaccharide mapping; Mass spectrometry; SNAIL INTERMEDIATE HOST; STRUCTURAL-CHARACTERIZATION; LINKED GLYCANS; FRESH-WATER; IDENTIFICATION; RESISTANT; DETERMINANTS; GLYCOLIPIDS; SPOROCYSTS; CERCARIAE;

D O I：

10.1016/j.exppara.2010.06.031

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Comparative analyses of the N-glycosylation pattern of hemolymph glycoproteins from Biomphalaria glabrata strains Puerto Rico (BgPR) and Salvador (BgBS-90), differing in their susceptibility towards Schistosoma mansoni infection, were performed by Western blotting, enzyme-linked immunosorbent assays, two-dimensional high-performance liquid chromatography and mass spectrometry. Obtained data demonstrated an enhanced expression of serologically cross-reacting, fucosylated carbohydrate epitopes by the highly susceptible BgPR-strain in comparison to the resistant BgBS-90-strain. In particular, glycoproteins of BgPR snails exhibited larger amounts of glycans with (beta 1-2)-linked xylose or terminal Fuc(alpha 1-3)GalNAc(beta 1-4)[+/- Fuc(alpha 1 -3)]GlcNAc(beta 1-)-units which are known to mediate cross-reactivity with schistosomal glycoconjugates. This finding could be corroborated by immunohistochemical studies showing again an enhanced expression of such carbohydrate epitopes in BgPR tissue. Hence, our results provide evidence for a correlation of B. glabrata susceptibility towards S. mansoni infection and the expression of carbohydrate determinants shared by the parasite and its intermediate host. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：592 / 602

页数：11

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