Photosynthetic Electron Transfer from Reaction Center Pigment-Protein Complex in Silica Nanopores

被引:31
|
作者
Oda, Ippei [1 ]
Iwaki, Masayo [2 ]
Fujita, Daiju [1 ]
Tsutsui, Yasutaka [1 ]
Ishizaka, Souji [1 ]
Dewa, Makiko [3 ]
Nango, Mamoru [3 ]
Kajino, Tsutomu [4 ]
Fukushima, Yoshiaki [4 ]
Itoh, Shigeru [1 ]
机构
[1] Nagoya Univ, Div Mat Sci Phys, Grad Sch Sci, Chikusa Ku, Aichi 4648602, Japan
[2] UCL, Glynn Lab Bioenerget, Dept Biol, London WC1E 6BT, England
[3] Nagoya Inst Technol, Dept Appl Chem, Grad Sch Engn, Nagoya, Aichi 4668555, Japan
[4] Toyota Cent Res & Dev Labs Inc, Aichi 4801192, Japan
关键词
BACTERIUM CHROMATIUM-TEPIDUM; PURPLE SULFUR BACTERIUM; MESOPOROUS SILICA; CHLOROPHYLL-A; THERMOCHROMATIUM-TEPIDUM; INFRARED-SPECTROSCOPY; CRYSTAL-STRUCTURES; MEMBRANE-PROTEIN; CYTOCHROME-C; ADSORPTION;
D O I
10.1021/la101810v
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A photosynthetic reaction center (RC) pigment-protein complex purified from a thermophilic purple photosynthetic bacterium, Thermochromatium tepidum, was adsorbed to a folded-sheet silica mesoporous material (FSM). The RC has a molecular structure with a 7.0 x 5.0 x 13 nm diameter. The amount of RC adsorbed to the FSM compound with an average internal pore diameter of 7.9 nm (FSM7.9) was high at 0.29 gRC/gFSM, while that to the FSM2.7 (2.7 nm diameter) was low at 0.02 gRC/gFSM, suggesting the specific binding of the RC into the 7.9 nm pores of FSM7.9. An N-2-adsorption isotherm study indicated the incorporation of the RC into the 7.9 nm pores. The RC inside FSM79. showed absorption spectra in the visible and infrared regions similar to those of the RC in solution, indicating almost no structural changes induced by the adsorption. The RC-FSM7.9 conjugate showed the high photochemical activity with the increased thermal stability up to 50 degrees C in the measurements by laser spectroscopy. The conjugates rapidly provided electrons to a dye in the outer medium or showed electric current on the ITO electrode upon the illumination. The RC-FSM conjugate will be useful for the construction of artificial photosynthetic systems and new photodevices.
引用
收藏
页码:13399 / 13406
页数:8
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