Environmental distribution and population biology of Candidatus Accumulibacter, a primary agent of biological phosphorus removal

被引:99
作者
Peterson, S. Brook [1 ]
Warnecke, Falk [2 ]
Madejska, Julita [2 ]
McMahon, Katherine D. [1 ,3 ]
Hugenholtz, Philip [2 ]
机构
[1] Univ Wisconsin, Microbiol Doctoral Training Program, Madison, WI 53706 USA
[2] DOE Joint Genome Inst, Microbial Ecol Program, Walnut Creek, CA USA
[3] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA
关键词
D O I
10.1111/j.1462-2920.2008.01690.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Members of the uncultured bacterial genus Candidatus Accumulibacter are capable of intracellular accumulation of inorganic phosphate in activated sludge wastewater treatment plants (WWTPs) performing enhanced biological phosphorus removal, but were also recently shown to inhabit freshwater and estuarine sediments. Additionally, metagenomic sequencing of two bioreactor cultures enriched in Candidatus Accumulibacter, but housed on separate continents, revealed the potential for global dispersal of particular Candidatus Accumulibacter strains, which we hypothesize is facilitated by the ability of Candidatus Accumulibacter to persist in environmental habitats. In the current study, we used sequencing of a phylogenetic marker, the ppk1 gene, to characterize Candidatus Accumulibacter populations in diverse environments, at varying distances from WWTPs. We discovered several new lineages of Candidatus Accumulibacter which had not previously been detected in WWTPs, and also uncovered new diversity and structure within previously detected lineages. Habitat characteristics were found to be a key determinant of Candidatus Accumulibacter lineage distribution while, as predicted, geographic distance played little role in limiting dispersal on a regional scale. However, on a local scale, enrichment of particular Candidatus Accumulibacter lineages in WWTP appeared to impact local environmental populations. These results provide evidence of ecological differences among Candidatus Accumulibacter lineages.
引用
收藏
页码:2692 / 2703
页数:12
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