In vivo evaluation of demyelination and remyelination in a nerve crush injury model

被引：61

作者：

Belanger, E. ^{[1
,2
]}

Henry, F. P. ^{[3
]}

Vallee, R. ^{[1
,2
]}

Randolph, M. A. ^{[3
]}

Kochevar, I. E. ^{[3
]}

Winograd, J. M. ^{[3
]}

Lin, C. P. ^{[3
]}

Cote, D. ^{[1
,2
]}

机构：

[1] Univ Laval, CRULRG, Quebec City, PQ G1J 2G3, Canada

[2] Univ Laval, COPL, Quebec City, PQ G1V 0A6, Canada

[3] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Boston, MA 02114 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 09期

基金：

加拿大自然科学与工程研究理事会;

关键词：

COHERENT; MYELIN; DEGENERATION; MECHANISMS; BIOLOGY;

D O I：

10.1364/BOE.2.002698

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nerves of the peripheral nervous system have, to some extent, the ability to regenerate after injury, particularly in instances of crush or contusion injuries. After a controlled crush injury of the rat sciatic nerve, demyelination and remyelination are followed with functional assessments and imaged both ex vivo and in vivo over the course of 4 weeks with video-rate coherent anti-Stokes Raman scattering (CARS) microscopy. A new procedure compatible with live animal imaging is developed for performing histomorphometry of myelinated axons. This allows quantification of demyelination proximal and remyelination distal to the crush site ex vivo and in vivo respectively. (C) 2011 Optical Society of America

引用

页码：2698 / 2708

页数：11

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