A highly sensitive fluorescent probe for the detection of bisulfite ion and its application in living cells

被引：40
作者：
Yang, Yutao [1 ]
Bai, Bozan [1 ]
Xu, Wenzhi [1 ]
Xu, Zhidong [1 ]
Zhang, Jinchao [1 ]
Li, Wei [1 ]
机构：
[1] Hebei Univ, Coll Chem & Environm Sci, Minist Educ, Key Lab Med Chem & Mol Diag, Baoding 071002, Peoples R China
来源：
DYES AND PIGMENTS | 2017年 / 136卷
基金：
美国国家科学基金会;
关键词：
Direct visualisation; Fluorescent; Bisulfite; Coumarin; TURN-ON PROBE; SO2; DERIVATIVES; SULFUR-DIOXIDE; 1,4-ADDITION REACTION; SELECTIVE DETECTION; AQUEOUS-SOLUTION; ASCORBIC-ACID; FAR-RED; SULFITE; WATER;
D O I：
10.1016/j.dyepig.2016.09.047
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A novel direct visualisation, fluorescent probe derived from coumarin for bisulfate ion detection has been designed and synthesized. This probe shows a rapid, high selectivity, and sensitivity in the detection of bisulfate ion. In a solution of DMSO:HEPES (1:9, v:v, 10 mM, pH 7.4) buffer containing the probe, the presence of HSO3- made the UV-vis absorption of the probe blue-shift from pink to yellow, and the fluorescence of the probe also changed from red to green. Other investigated analytes and ions did not disturb the determination of HSO3-. The reaction mechanism of the determination was proposed based on the nucleophilic attack toward electron-poor C = C bond of the probe. This probe has been successfully applied for determination of HSO3- in living cells. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：830 / 835
页数：6
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