A highly sensitive fluorescent probe for the detection of bisulfite ion and its application in living cells

被引：40

作者：

Yang, Yutao ^{[1
]}

Bai, Bozan ^{[1
]}

Xu, Wenzhi ^{[1
]}

Xu, Zhidong ^{[1
]}

Zhang, Jinchao ^{[1
]}

Li, Wei ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Minist Educ, Key Lab Med Chem & Mol Diag, Baoding 071002, Peoples R China

来源：

DYES AND PIGMENTS | 2017年 / 136卷

基金：

美国国家科学基金会;

关键词：

Direct visualisation; Fluorescent; Bisulfite; Coumarin; TURN-ON PROBE; SO2; DERIVATIVES; SULFUR-DIOXIDE; 1,4-ADDITION REACTION; SELECTIVE DETECTION; AQUEOUS-SOLUTION; ASCORBIC-ACID; FAR-RED; SULFITE; WATER;

D O I：

10.1016/j.dyepig.2016.09.047

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel direct visualisation, fluorescent probe derived from coumarin for bisulfate ion detection has been designed and synthesized. This probe shows a rapid, high selectivity, and sensitivity in the detection of bisulfate ion. In a solution of DMSO:HEPES (1:9, v:v, 10 mM, pH 7.4) buffer containing the probe, the presence of HSO3- made the UV-vis absorption of the probe blue-shift from pink to yellow, and the fluorescence of the probe also changed from red to green. Other investigated analytes and ions did not disturb the determination of HSO3-. The reaction mechanism of the determination was proposed based on the nucleophilic attack toward electron-poor C = C bond of the probe. This probe has been successfully applied for determination of HSO3- in living cells. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：830 / 835

页数：6

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