DddW, a third DMSP lyase in a model Roseobacter marine bacterium, Ruegeria pomeroyi DSS-3

被引：80

作者：

Todd, Jonathan D. ^{[1
]}

Kirkwood, Mark ^{[1
]}

Newton-Payne, Simone ^{[1
]}

Johnston, Andrew W. B. ^{[1
]}

机构：

[1] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England

来源：

ISME JOURNAL | 2012年 / 6卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

gene regulation; dimethyl sulfide; DMSP; Roseobacters; Ruegeria pomeroyi; DIMETHYL SULFIDE; DIMETHYLSULFONIOPROPIONATE LYASE; SULFUR; OCEAN; GENE; PROTEOBACTERIA; CLIMATE;

D O I：

10.1038/ismej.2011.79

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Ruegeria pomeroyi DSS-3 is a model Roseobacter marine bacterium, particularly regarding its catabolism of dimethylsulfoniopropionate (DMSP), an abundant anti-stress molecule made by marine phytoplankton. We found a novel gene, dddW, which encodes a DMSP lyase that cleaves DMSP into acrylate plus the environmentally important volatile dimethyl sulfide (DMS). Mutations in dddW reduced, but did not abolish DMS production. Transcription of dddW was greatly enhanced by pre-growth of cells with DMSP, via a LysR-type regulator. Close DddW homologs occur in only one other Roseobacter species, and there are no close homologs and only a few related sequences in metagenomes of marine bacteria. In addition to DddW, R. pomeroyi DSS-3 had been shown to have two other, different, DMSP lyases, DddP and DddQ, plus an enzyme that demethylates DMSP, emphasizing the importance of this substrate for this model bacterium. The ISME Journal (2012) 6, 223-226; doi:10.1038/ismej.2011.79; published online 16 June 2011

引用

页码：223 / 226

页数：4

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