Knockdown of Diaph1 expression inhibits migration and decreases the expression of MMP2 and MMP9 in human glioma cells

被引：23

作者：

Zhang, Can ^{[1
]}

Wang, Lin ^{[1
]}

Chen, Jiaying ^{[1
]}

Liang, Jie ^{[1
]}

Xu, Yaming ^{[1
]}

Li, Zhe ^{[1
]}

Chen, Fuxue ^{[1
]}

Du, Dongshu ^{[1
]}

机构：

[1] Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2017年 / 96卷

关键词：

Diaph1; Glioma; Migration; Matrix metalloproteinases; PROSTATE-CANCER; INVADOPODIA FORMATION; INVASION; IDENTIFICATION; CADHERIN; GENE; ECM;

D O I：

10.1016/j.biopha.2017.10.031

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

As the most common primary central nervous system tumor, glioma is characterized by high levels of mortality and migration. Unclear boundary with normal brain tissue results in poor treatment. The mammalian diaphanous-related formin 1 (Diaph1) which belongs to formin-homology protein family, is a target of RhoA and involved in a number of actin-related biological processes, which abnormally expressed in pathological conditions in a number of tumors. Immunohistochemical analysis showed that Diaph1 was overexpressed in glioma tissues compared with normal human brain tissue. Diaph1 gene silencing RNA interference (RNAi) significantly inhibited the migratory activity of human glioma cell lines U87 and U251. Moreover, data obtained from qRT-PCR and Western-blot analysis showed that the mRNA and protein expression of matrix metalloproteinase2 and 9 (MMP2 and MMP9) was significantly suppressed in these Diaph1 knockdown cell lines, as well as gelatin zymography analysis revealed that the activity of MMP2 and MMP9 in conditioned medium was markedly decreased. In conclusion, our data demonstrate that Diaph1 is highly expressed in human glioma, plays a significant role in glioma cell migration, and can influence the expression and activity of MMP2 and MMP9 indirectly in human glioma cell lines U87 and U251. We provide a theoretical basis for further experimental studies and Diaph1 using on glioma therapy.

引用

页码：596 / 602

页数：7

共 27 条

[1] Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain [J].

Alberts, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (04) :2824-2830

[2] Small GTPase Rho regulates R-cadherin through Dia1/profilin-1 [J].

Bonacci, Tabetha M. ;

Hirsch, Dianne S. ;

Shen, Yi ;

Dokmanovic, Milos ;

Wu, Wen Jin .

CELLULAR SIGNALLING, 2012, 24 (11) :2102-2110

[3] Newly codified glial neoplasms of the 2007 WHO Classification of Tumours of the Central Nervous System: Angiocentric glioma, pilomyxoid astrocytoma and pituicytoma [J].

Brat, Daniel J. ;

Scheithauer, Bernd W. ;

Fuller, Gregory N. ;

Tihan, Tarik .

BRAIN PATHOLOGY, 2007, 17 (03) :319-324

[4] Role of tenascins in the ECM of gliomas [J].

Broesicke, Nicole ;

Faissner, Andreas .

CELL ADHESION & MIGRATION, 2015, 9 (1-2) :131-140

[5] Mammalian diaphanous-related formin Dia1 controls the organization of E-cadherin-mediated cell-cell junctions [J].

Carramusa, Letizia ;

Ballestrem, Christoph ;

Zilberman, Yuliya ;

Bershadsky, Alexander D. .

JOURNAL OF CELL SCIENCE, 2007, 120 (21) :3870-3882

[6]

CASTRILLON DH, 1994, DEVELOPMENT, V120, P3367

[7] Invadopodia and matrix degradation, a new property of prostate cancer cells during migration and invasion [J].

Desai, Bhavik ;

Ma, Tao ;

Chellaiah, Meenakshi A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (20) :13856-13866

[8] Surgical removal of corpus callosum infiltrated by low-grade glioma: functional outcome and oncological considerations [J].

Duffau, H ;

Khalil, I ;

Gatignol, P ;

Denvil, D ;

Capelle, L .

JOURNAL OF NEUROSURGERY, 2004, 100 (03) :431-437

[9] Active site specificity profiling datasets of matrix metalloproteinases (MMPs) 1, 2, 3, 7, 8, 9, 12, 13 and 14 [J].

Eckhard, Ulrich ;

Huesgen, Pitter F. ;

Schilling, Oliver ;

Bellac, Caroline L. ;

Butler, Georgina S. ;

Cox, Jennifer H. ;

Dufour, Antoine ;

Goebeler, Verena ;

Kappelhoff, Reinhild ;

auf dem Keller, Ulrich ;

Klein, Theo ;

Lange, Philipp F. ;

Marino, Giada ;

Morrison, Charlotte J. ;

Prudova, Anna ;

Rodriguez, David ;

Starr, Amanda E. ;

Wang, Yili ;

Overall, Christopher M. .

DATA IN BRIEF, 2016, 7 :299-310

[10] GPNMB/OA protein increases the invasiveness of human metastatic prostate cancer cell lines DU145 and PC3 through MMP-2 and MMP-9 activity [J].

Fiorentini, Chiara ;

Bodei, Serena ;

Bedussi, Francesca ;

Fragni, Martina ;

Bonini, Sara Anna ;

Simeone, Claudio ;

Zani, Danilo ;

Berruti, Alfredo ;

Missale, Cristina ;

Memo, Maurizio ;

Spano, PierFranco ;

Sigala, Sandra .

EXPERIMENTAL CELL RESEARCH, 2014, 323 (01) :100-111

← 1 2 3 →