Cladophora glomerata methanolic extract decreases oxidative stress and improves viability and mitochondrial potential in equine adipose derived mesenchymal stem cells (ASCs)

被引:27
作者
Bourebaba, Lynda [1 ]
Michalak, Izabela [2 ]
Roecken, Michael [3 ]
Marycz, Krzysztof [1 ,3 ]
机构
[1] Wroclaw Univ Environm & Life Sci, Fac Biol & Anim Sci, Dept Expt Biol, Norwida 27B, PL-50375 Wroclaw, Poland
[2] Wroclaw Univ Sci & Technol, Fac Chem, Dept Adv Mat Technol, Smoluchowskiego 25, PL-50372 Wroclaw, Poland
[3] Justus Liebig Univ, Equine Clin Equine Surg, Fac Vet Med, D-35392 Giessen, Germany
关键词
Cladophora glomerata; EMS; ASCs; Oxidative stress; Mitopotential; Inflammation; ENDOPLASMIC-RETICULUM STRESS; HYDROGEN-PEROXIDE; ANTIOXIDANT ENZYMES; FREE-RADICALS; AMINO-ACIDS; FATTY-ACIDS; APOPTOSIS; DAMAGE; DYSFUNCTION; MACROALGAE;
D O I
10.1016/j.biopha.2018.12.020
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Reactive oxygen species (ROS) are key mediators of several cellular damage and thus associated with equine diseases such as inflammation and metabolic syndrome. This study aimed to evaluate the protective and antioxidant activities of methanolic extract prepared from Cladophora glomerata (C. glomerata) biomass, on equine adipose derived mesenchymal stem cells (EqASCs), under experimental oxidative stress induced by H2O2. Pretreatment of EqASCs cells with different concentrations of C. glomerata methanolic extract (1% and 5%) provided a clear protection against cellular damage triggered by H2O2. The cell's apoptotic status was significantly regulated, with promotion of cell viability, down-regulation of pro-apoptotic (p21, p53, Bax and Casp-9) genes expression, concomitant to up-regulation of the survival gene Bcl-2, this being supported by a mitigation of the endoplasmic reticulum (ER) stress and significant minimization of mitochondrial dysfunction. The results also showed that C. glomerata extract significantly increased the antioxidant enzymes Superoxide dismutase (SOD) and Catalase (CAT) activities, positively regulated the enzymes genes expression, and markedly reduced the protein carbonyls derivatives production. Finally, RT-qPCR analysis of the inflammatory related genes allowed to highlight a promising anti-inflammatory and immunomodulatory effect of this extract. Due to the valuable antioxidant and anti-inflammatory activities, C. glomerata may have potential benefits for the prevention of equine diseases associated with oxidative stress, including metabolic syndrome.
引用
收藏
页码:6 / 18
页数:13
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