Polyploidy Affects Plant Growth and Alters Cell Wall Composition

被引：161

作者：

Corneillie, Sander ^{[1
,2
]}

De Storme, Nico ^{[3
]}

Van Acker, Rebecca ^{[1
,2
]}

Fangel, Jonatan U. ^{[4
]}

De Bruyne, Michiel ^{[1
,2
]}

De Rycke, Riet ^{[1
,2
]}

Geelen, Danny ^{[3
]}

Willats, William G. T. ^{[5
,6
]}

Vanholme, Bartel ^{[1
,2
]}

Boerjan, Wout ^{[1
,2
]}

机构：

[1] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[2] VIB, VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium

[3] Univ Ghent, Fac Biosci Engn, Dept Plant Prod, B-9000 Ghent, Belgium

[4] Carlsberg Res Labs, DK-1799 Copenhagen V, Denmark

[5] Univ Copenhagen, Dept Biol, DK-2200 Copenhagen, Denmark

[6] Newcastle Univ, Sch Agr Food & Rural Dev, Agr Bldg, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

来源：

PLANT PHYSIOLOGY | 2019年 / 179卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

LIGNIN BIOSYNTHETIC-PATHWAY; GENOME; MUTANT; DEPOSITION; EXPRESSION; ENZYME; POPLAR; HYBRID; YIELD; GRASS;

D O I：

10.1104/pp.18.00967

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Polyploidization has played a key role in plant breeding and crop improvement. Although its potential to increase biomass yield is well described, the effect of polyploidization on biomass composition has largely remained unexplored. Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization. Kinematic analysis showed that polyploids developed slower compared to diploids; however, tetra-and hexaploids, but not octaploids, generated larger rosettes due to delayed flowering. In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy. However, the inflorescence stem dry weight was highest in tetraploids. Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue. In addition, higher ploidy plants displayed altered sugar composition. Such effects were linked to the delayed development of polyploids. Moreover, the changes in polyploid cell wall composition promoted saccharification yield. The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.

引用

页码：74 / 87

页数：14

共 57 条

[31] p-Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon [J].