Identification and cloning of three novel human G protein-coupled receptor genes GPR52, ΨGPR53 and GPR55:: GPR55 is extensively expressed in human brain

被引:293
作者
Sawzdargo, M
Nguyen, T
Lee, DK
Lynch, KR
Cheng, R
Heng, HHQ
George, SR
O'Dowd, BF
机构
[1] Univ Toronto, Dept Pharmacol, Toronto, ON, Canada
[2] Univ Virginia, Hlth Sci Ctr, Dept Pharmacol, Charlottesville, VA 22908 USA
[3] See DNA Biotech, N York, ON M3J 1P3, Canada
[4] Ctr Addict & Mental Hlth, Toronto, ON M5S 2S1, Canada
来源
MOLECULAR BRAIN RESEARCH | 1999年 / 64卷 / 02期
基金
英国医学研究理事会;
关键词
receptor; GPCR; intronless; pseudogene; chromosome; northern blot;
D O I
10.1016/S0169-328X(98)00277-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The G protein-coupled receptor (GPCR) family share a structural motif of seven transmembrane segments with large numbers of conserved residues in those regions. Here, we report the identification and cloning of two novel human intronless GPCR genes, GPR52, GPR55 and a pseudogene Psi GPR53. GPR55 was identified from the expressed sequence tags (EST) database whereas GPR52 and pseudogene Psi GPR53 originated from the high throughput genome (HTG) database. A partial cDNA clone obtained from the IMAGE Consortium of GPR55 was used to screen a human genomic library to acquire the full length gene. GPR52 and Psi GPR53 were amplified from human genomic DNA using primers based on the HTG sequences. GPR55 and GPR52 encode receptors of 319 and 361 amino acids, respectively. GPR55 gene was mapped to chromosome 2q37, using fluorescence in situ hybridization (FISH), and its mRNA transcripts have been detected in the caudate nucleus and putamen, but not in five other brain regions. Human receptors showing the highest amino acid identity to GPR55 include P2Y5 (29%), GPR23 (30%), GPR35 (27%) and CCR4 (23%). GPR52 gene localized to chromosome 1q24 shares the highest identity with GPR21 (71%), histamine H2 (27%) and 5-HT4 (26%) human receptors. Psi GPR53 is a pseudogene mapped to chromosome 6p21 that demonstrates the highest similarity to the MRG (35%), MAS (28%) and C5a (24%) human receptor genes. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:193 / 198
页数:6
相关论文
共 13 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[3]   TOWARDS A PARADIGM SHIFT IN BIOLOGY [J].
GILBERT, W .
NATURE, 1991, 349 (6305) :99-99
[4]   MODES OF DAPI BANDING AND SIMULTANEOUS INSITU HYBRIDIZATION [J].
HENG, HHQ ;
TSUI, LC .
CHROMOSOMA, 1993, 102 (05) :325-332
[5]   HIGH-RESOLUTION MAPPING OF MAMMALIAN GENES BY INSITU HYBRIDIZATION TO FREE CHROMATIN [J].
HENG, HHQ ;
SQUIRE, J ;
TSUI, LC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) :9509-9513
[6]  
KENNELLY PJ, 1991, J BIOL CHEM, V266, P15555
[7]  
KOZAK M, 1991, J BIOL CHEM, V266, P19867
[8]   Interpreting cDNA sequences: Some insights from studies on translation [J].
Kozak, M .
MAMMALIAN GENOME, 1996, 7 (08) :563-574
[9]   CLONING OF HUMAN GENES ENCODING NOVEL G-PROTEIN-COUPLED RECEPTORS [J].
MARCHESE, A ;
DOCHERTY, JM ;
NGUYEN, T ;
HEIBER, M ;
CHENG, R ;
HENG, HHQ ;
TSUI, LC ;
SHI, XM ;
GEORGE, SR ;
ODOWD, BF .
GENOMICS, 1994, 23 (03) :609-618
[10]  
ODOWD BF, 1989, J BIOL CHEM, V264, P7564