Virulence determinants between New York 99 and Kunjin strains of West Nile virus

被引：42

作者：

Audsley, Michelle ^{[1
]}

Edmonds, Judith ^{[1
]}

Liu, Wenjun ^{[1
]}

Mokhonov, Vlad ^{[1
]}

Mokhonova, Ekaterina ^{[1
]}

Melian, Ezequeil Balmori ^{[1
]}

Prow, Natalie ^{[1
]}

Hall, Roy A. ^{[1
]}

Khromykh, Alexander A. ^{[1
]}

机构：

[1] Univ Queensland, Ctr Infect Dis Res, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

VIROLOGY | 2011年 / 414卷 / 01期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

Flavivirus; West Nile virus; Kunjin virus; Virulence factors; Infectious clone; Chimeric viruses; AMINO-ACID SUBSTITUTION; NY99; STRAIN; PROTEIN; PHENOTYPE; MOUSE; MICE; NS5; TRANSCOMPLEMENTATION; REPLICATION; MUTATIONS;

D O I：

10.1016/j.virol.2011.03.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An attenuated Australian strain of West Nile virus (WNV), Kunjin (KUN), shares similar to 98% amino acid homology with the pathogenic New York 99 NY99 strain (NY99). To investigate the viral factors involved in NY99 virulence we generated an infectious cDNA clone of the WNV NY99 4132 isolate from which virus was recovered and was shown to be indistinguishable from the parental isolate. We then introduced the regions of the NY99 non-structural (NS) proteins and/or untranslated regions (UTRs) into the KUN backbone. Chimeric KUN viruses containing NY99 5'UTR and the parts of NS coding region were more virulent in mice than parental KUN virus. Chimeric NY99 viruses, containing KUN NS2A protein with alanine 30 to proline substitution were significantly less cytopathic in cells and less virulent in mice. Our results identify the 5'UTR and NS proteins as WNV virulence determinants and confirm a role for the NS2A in WNV cytopathicity and virulence. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：63 / 73

页数：11

共 23 条

[1] Envelope protein glycosylation status influences mouse neuroinvasion phenotype of genetic lineage 1 West Nile Virus strains [J].