Virus isolation and molecular characterization of canine distemper virus by RT-PCR from a mature dog with multifocal encephalomyelitis

被引：7

作者：

Amude, Alexandre Mendes ^{[1
]}

Carvalho, Glei Dos Anjos ^{[1
]}

Alfieri, Amauri Alcindo ^{[1
]}

Alfieri, Alice Fernandes ^{[1
]}

机构：

[1] Univ Estadual Londrina, Lab Virol Anim, CCA, Dept Vet Prevent Med, BR-86051090 Londrina, Parana, Brazil

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2007年 / 38卷 / 02期

关键词：

dog; canine distemper virus; encephalomyelitis; RT-PCR;

D O I：

10.1590/S1517-83822007000200031

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A case of multifocal distemper encephalomyelitis in a mature dog is described. In the presented case the ante mortem clinical diagnosis of canine distemper virus (CDV) infection could not be ideally performed due to the absence of typical signs of distemper, such as myoclonus and systemic signs accompanying the nervous signs. The definitive diagnosis of distemper encephalomyelitis was only carried out at post mortem through virus isolation in cell culture from fresh central nervous system (CNS) fragments and CDV nucleoprotein gene detection in the CNS by RT-PCR.

引用

收藏

页码：354 / 356

页数：3

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