Role of Hexahistidine in Directed Nanoassemblies of Tobacco Mosaic Virus Coat Protein

被引：61

作者：

Bruckman, Michael A. ^{[1
]}

Soto, Carissa M. ^{[1
]}

McDowell, Heather ^{[1
]}

Liu, Jinny L. ^{[1
]}

Ratna, Banahalli R. ^{[1
]}

Korpany, Katalin V. ^{[2
]}

Zahr, Omar K. ^{[2
]}

Blum, Amy Szuchmacher ^{[2
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

来源：

ACS NANO | 2011年 / 5卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

tobacco mosaic virus; atomic force microscopy; self-assembly; transmission electron microscopy; His-tagged protein; Escherichia coli; METAL CHELATE ADSORBENT; ANILINE POLYMERIZATION; SURFACE MODIFICATION; NANOSTRUCTURES; NANOTECHNOLOGY; NANOPARTICLES; PURIFICATION; ASSEMBLIES; TEMPLATES; BOTTOM;

D O I：

10.1021/nn1025719

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A common challenge in nanotechnology is the fabrication of materials with well-defined nanoscale structure and properties. Here we report that a genetically engineered tobacco mosaic virus (TMV) coat protein (CP), to which a hexahistidine (His) tag was incorporated, can self-assemble Into disks, hexagonally packed arrays of disks, stacked disks, helical rods, fibers, and elongated rafts. The insertion of a His tag to the C-terminus of TMV-CP was shown to significantly affect the self-assembly In comparison to the wild type, WT-TMV-CP. Furthermore, the His tag Interactions attributed to the alternative self assembly of His-TMV-CP can be controlled through ethanol and nickel-nitrilotriacetic acid, (Ni-NTA) additions as monitored with atomic force microscopy.

引用

页码：1606 / 1616

页数：11

共 50 条

[21] β-structure of the coat protein subunits in spherical particles generated by tobacco mosaic virus thermal denaturation [J].

Dobrov, Evgeny N. ;

Nikitin, Nikolai A. ;

Trifonova, Ekaterina A. ;

Parshina, Evgenia Yu. ;

Makarov, Valentin V. ;

Maksimov, George V. ;

Karpova, Olga V. ;

Atabekov, Joseph G. .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2014, 32 (05) :701-708

[22] THE MECHANISM(S) OF COAT PROTEIN-MEDIATED RESISTANCE AGAINST TOBACCO MOSAIC-VIRUS [J].

REIMANNPHILIPP, U ;

BEACHY, RN .

SEMINARS IN VIROLOGY, 1993, 4 (06) :349-356

[23] Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors [J].

Baecker, Matthias ;

Koch, Claudia ;

Eiben, Sabine ;

Geiger, Fania ;

Eber, Fabian ;

Gliemann, Hartmut ;

Poghossian, Arshak ;

Wege, Christina ;

Schoening, Michael J. .

SENSORS AND ACTUATORS B-CHEMICAL, 2017, 238 :716-722

[24] A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection [J].

Zhao, MM ;

An, DR ;

Huang, GH ;

He, ZH ;

Chen, JY .

ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2005, 37 (04) :248-253

[25] A Viral Protein Suppresses siRNA-directed Interference in Tobacco Mosaic Virus Infection [J].

Ming-Min ZHAO De-Rong AN Guang-Hua HUANG~1 Zu-Hua HE~2 Jiang-Ye CHEN~1College of Plant Protection ;

~1State Key Laboratory of Molecular Biology ;

~2State Key Laboratory of Plant Molecular Genetics.Institute of Plant Physiology and Ecology .

Acta Biochimica et Biophysica Sinica, 2005, (04) :248-253

[26] New Strategies and Methods to Study Interactions between Tobacco Mosaic Virus Coat Protein and Its Inhibitors [J].

Li, Xiangyang ;

Chen, Zhuo ;

Jin, Linhong ;

Hu, Deyu ;

Yang, Song .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (03)

[27] A point mutation in the coat protein gene affects long-distance transport of the tobacco mosaic virus [J].

Koshkina, TE ;

Baranova, EN ;

Usacheva, EA ;

Zavriev, SK .

MOLECULAR BIOLOGY, 2003, 37 (04) :631-636

[28] Protein-Embedded Metalloporphyrin Arrays Templated by Circularly Permuted Tobacco Mosaic Virus Coat Proteins [J].

Dai, Jing ;

Knott, Gavin J. ;

Fu, Wen ;

Lin, Tiffany W. ;

Furst, Ariel L. ;

Britt, R. David ;

Francis, Matthew B. .

ACS NANO, 2021, 15 (05) :8110-8119

[29] Functionalized Tobacco Mosaic Virus Coat Protein Monomers and Oligomers as Nanocarriers for Anti-Cancer Peptides [J].

Gamper, Coralie ;

Spenle, Caroline ;

Bosca, Sonia ;

van der Heyden, Michael ;

Erhardt, Mathieu ;

Orend, Gertraud ;

Bagnard, Dominique ;

Heinlein, Manfred .

CANCERS, 2019, 11 (10)

[30] A Point Mutation in the Coat Protein Gene Affects Long-Distance Transport of the Tobacco Mosaic Virus [J].

T. E. Koshkina ;

E. N. Baranova ;

E. A. Usacheva ;

S. K. Zavriev .

Molecular Biology, 2003, 37 :631-636

← 1 2 3 4 5 →