Role of Hexahistidine in Directed Nanoassemblies of Tobacco Mosaic Virus Coat Protein

被引：63

作者：

Bruckman, Michael A. ^{[1
]}

Soto, Carissa M. ^{[1
]}

McDowell, Heather ^{[1
]}

Liu, Jinny L. ^{[1
]}

Ratna, Banahalli R. ^{[1
]}

Korpany, Katalin V. ^{[2
]}

Zahr, Omar K. ^{[2
]}

Blum, Amy Szuchmacher ^{[2
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

来源：

ACS NANO | 2011年 / 5卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

tobacco mosaic virus; atomic force microscopy; self-assembly; transmission electron microscopy; His-tagged protein; Escherichia coli; METAL CHELATE ADSORBENT; ANILINE POLYMERIZATION; SURFACE MODIFICATION; NANOSTRUCTURES; NANOTECHNOLOGY; NANOPARTICLES; PURIFICATION; ASSEMBLIES; TEMPLATES; BOTTOM;

D O I：

10.1021/nn1025719

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A common challenge in nanotechnology is the fabrication of materials with well-defined nanoscale structure and properties. Here we report that a genetically engineered tobacco mosaic virus (TMV) coat protein (CP), to which a hexahistidine (His) tag was incorporated, can self-assemble Into disks, hexagonally packed arrays of disks, stacked disks, helical rods, fibers, and elongated rafts. The insertion of a His tag to the C-terminus of TMV-CP was shown to significantly affect the self-assembly In comparison to the wild type, WT-TMV-CP. Furthermore, the His tag Interactions attributed to the alternative self assembly of His-TMV-CP can be controlled through ethanol and nickel-nitrilotriacetic acid, (Ni-NTA) additions as monitored with atomic force microscopy.

引用

页码：1606 / 1616

页数：11

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