Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology

被引：15

作者：

Chen, Yi-Cheng ^{[1
]}

Tsai, Pi-Ju ^{[2
]}

Huang, Yaw-Bin ^{[1
]}

Wu, Pao-Chu ^{[1
]}

机构：

[1] Kaohsiung Med Univ, Coll Pharm, Sch Pharm, Kaohsiung, Taiwan

[2] I Shou Univ, Dept Business Adm, Kaohsiung, Taiwan

来源：

PLOS ONE | 2015年 / 10卷 / 03期

关键词：

MILD ACNE-VULGARIS; RANDOMIZED-TRIAL; TRETINOIN GEL; EFFICACY; SAFETY; TOLERABILITY; FORMULATION; HPLC; ACID;

D O I：

10.1371/journal.pone.0120171

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to develop a simple and reliable high-performance chromatographic (HPLC) method for simultaneous analysis of adapalene and benzoyl peroxide in pharmaceutical formulation by response surface methodology (RSM). An optimized mobile phase composed of acetonitrile, tetrahydrofuran and water containing 0.1% acetic acid at a ratio of 25: 50: 25 by volume was successfully predicted by using RSM. An isocratic separation was achieved by using the condition. Furthermore, the analytical method was validated in terms of specificity, linearity, accuracy and precision in a range of 80% to 120% of the expected concentration. Finally, the method was successfully applied to the analysis of a commercial product.

引用

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