Super-resolution imaging for monitoring cytoskeleton dynamics

被引：13

作者：

Finkenstaedt-Quinn, Solaire A. ^{[1
,2
,3
]}

Qiu, Tian A. ^{[1
]}

Shin, Kayeong ^{[1
]}

Haynes, Christy L. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Chem, 207 Pleasant St SE, Minneapolis, MN 55455 USA

[2] Univ Michigan, Dept Chem, 930 N Univ St, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Sweetland Ctr Writing, 930 N Univ St, Ann Arbor, MI 48109 USA

来源：

ANALYST | 2016年 / 141卷 / 20期

基金：

美国国家科学基金会;

关键词：

LIVE-CELL; ATOMIC-FORCE; ACTIN POLYMERIZATION; MICROTUBULE DYNAMICS; PLASMA-MEMBRANE; INTERMEDIATE-FILAMENTS; FLUORESCENT-PROBES; DIFFRACTION-LIMIT; TIRF MICROSCOPY; MYOSIN-V;

D O I：

10.1039/c6an00731g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The cytoskeleton is a key cellular structure that is important in the control of cellular movement, structure, and sensing. To successfully image the individual cytoskeleton components, high resolution and super-resolution fluorescence imaging methods are needed. This review covers the three basic cytoskeletal elements and the relative benefits and drawbacks of fixed versus live cell imaging before moving on to recent studies using high resolution and super-resolution techniques. The techniques covered include the near-diffraction limited imaging methods of confocal microscopy and TIRF microscopy and the super-resolution fluorescence imaging methods of STORM, PALM, and STED.

引用

页码：5674 / 5688

页数：15

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