Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes

被引：4
作者：
Gu, Yingying
Li, Qingge [1 ]
机构：
[1] Xiamen Univ, Mol Diagnost Lab, Dept Biomed Sci, Sch Life Sci, Xiamen 361005, Fujian, Peoples R China
[2] Xiamen Univ, State Key Lab Minist Educ Cell Biol & Tumor Cell, Sch Life Sci, Xiamen 361005, Fujian, Peoples R China
来源：
CLINICAL BIOCHEMISTRY | 2007年 / 40卷 / 16-17期
关键词：
aldehyde dehydrogenase-2; genotyping; real-time PCR; displacing probes;
D O I：
10.1016/j.clinbiochem.2007.07.009
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping. Design and methods: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes. Results: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis. Conclusions: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2. (C) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：1325 / 1327
页数：3
相关论文
共 10 条
  • [1] Real-time PCR genotyping using displacing probes
    Cheng, JP
    Zhang, YY
    Li, QG
    [J]. NUCLEIC ACIDS RESEARCH, 2004, 32 (07) : e61
  • [2] Rapid genotyping of single nucleotide polymorphisms using novel minor groove binding DNA oligonucleotides (MGB probes)
    de Kok, JB
    Wiegerinck, ETG
    Giesendorf, BAJ
    Swinkels, DW
    [J]. HUMAN MUTATION, 2002, 19 (05) : 554 - 559
  • [3] HARADA S, 1993, ALCOHOL ALCOHOLISM, V28, P11
  • [4] Comparative analyses of four different methods of genotyping ALDH2
    Itoga, S
    Nanmoku, T
    Uchimoto, T
    Sunaga, M
    Nezu, M
    Tomonaga, T
    Harada, S
    Nomura, F
    [J]. ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2004, 28 (08) : 117S - 122S
  • [5] A new class of homogeneous nucleic acid probes based on specific displacement hybridization
    Li, QQ
    Luan, GY
    Guo, QP
    Liang, JX
    [J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (02) : 5
  • [6] Marras Salvatore A E, 2003, Methods Mol Biol, V212, P111
  • [7] Assessment of a difference in ALDH2 heterozygotes and alcoholic liver injury
    Nagata, N
    Hiyoshi, M
    Shiozawa, H
    Shiraishi, K
    Watanabe, N
    Tsuda, M
    Matsuzaki, S
    [J]. ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2002, 26 (08) : 11S - 14S
  • [8] Maternal inheritance of diabetes is associated with inactive ALDH2 genotype in diabetics with renal failure in Japanese
    Suzuki, Y
    Kuriyama, S
    Atsumi, Y
    Murata, C
    Matsuoka, K
    Suzuki, Y
    Taniyama, M
    Muramatsu, T
    Suzuki, Y
    Ohta, S
    [J]. DIABETES RESEARCH AND CLINICAL PRACTICE, 2003, 60 (02) : 143 - 145
  • [9] Alcohol-related cancers and aldehyde dehydrogenase-2 in Japanese alcoholics
    Yokoyama, A
    Muramatsu, T
    Ohmori, T
    Yokoyama, T
    Okuyama, K
    Takahashi, H
    Hasegawa, Y
    Higuchi, S
    Maruyama, K
    Shirakura, K
    Ishii, H
    [J]. CARCINOGENESIS, 1998, 19 (08) : 1383 - 1387
  • [10] YOSHIDA A, 1983, AM J HUM GENET, V35, P1107
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