Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy

被引：17

作者：

Park, Soongho ^{[1
]}

Nguyen, Thien ^{[1
]}

Benoit, Emilie ^{[2
]}

Sackett, D. A. N. L. ^{[1
]}

Garmendia-Cedillos, Marcial ^{[3
]}

Pursley, Randall ^{[3
]}

Boccara, Claude ^{[2
,4
]}

Gandjbakhche, Amir ^{[1
]}

机构：

[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, 49 Convent Dr, Bethesda, MD 20814 USA

[2] LLTech SAS Aquyre Biosci, 58 Rue Dessous Berges, F-75013 Paris, France

[3] NIH, Signal Proc & Instrumentat Sect, Ctr Informat Technol, 49 Convent Dr, Bethesda, MD 20814 USA

[4] PSL Univ, ESPCI Paris, CNRS, Inst Langevin, 1 Rue Jussieu, F-75005 Paris, France

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 10期

基金：

美国国家卫生研究院;

关键词：

GREEN FLUORESCENT PROTEIN; APOPTOSIS; PROBES; CULTURE; CANCER; ULTRASOUND; DIAGNOSIS; SYSTEMS; DNA;

D O I：

10.1364/BOE.436330

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79 +/- 0.5 Hz and 2.44 +/- 1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57 +/- 0.71 Hz, whereas the magnitude was significantly decreased to 0.53 +/- 0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：6431 / 6441

页数：11

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