EVALUATION OF A COMMERCIAL COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF AVIAN INFLUENZA VIRUS SUBTYPE H5 ANTIBODIES IN ZOO BIRDS

被引：3

作者：

Jensen, Trine Hammer ^{[1
,2
,4
]}

Andersen, Jannie Holmegaard ^{[2
]}

Hjulsager, Charlotte Kristiane ^{[1
]}

Chriel, Mariann ^{[1
]}

Bertelsen, Mads Frost ^{[3
]}

机构：

[1] Tech Univ Denmark, Natl Vet Inst, Bulowsvej 27, DK-1870 Frederiksberg C, Denmark

[2] Aalborg Univ, Dept Chem & Biosci, Fredrik Bajers Vej 7H, DK-9000 Aalborg, Denmark

[3] Copenhagen Zoo, Ctr Zoo & Wild Anim Hlth, Roskildevej 38, DK-2000 Frederiksberg, Denmark

[4] Aalborg Univ, Aalborg Zoo, Molleparkvej 63, DK-9000 Aalborg, Denmark

来源：

JOURNAL OF ZOO AND WILDLIFE MEDICINE | 2017年 / 48卷 / 03期

关键词：

Avian influenza; ELISA; H5; hemagglutination inhibition test; zoo birds; VACCINATION;

D O I：

10.1638/2016-0220.1

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The hemagglutination inhibition (HI) test is the current gold standard for detecting antibodies to avian influenza virus (AIV). Enzyme-linked immunosorbent assays (ELISAs) have been explored for use in poultry and certain wild bird species because of high efficiency and lower cost. This study compared a commercial ELISA for detection of AIV subtype Hi antibodies with x(2) test of 572 serum samples from zoo birds. There was no significant difference between the results of the two tests when statistically compared by a McNemar v2 test (P = 0.86) and assessment of k (k = 0.87). With a specificity of 94.2% (95% confidence interval [CI], 0.92-0.97), a sensitivity of 93.9% (95% CI, 0.91-0.97), and an excellent correlation between the two tests, this ELISA can be recommended as an alternative to the x(2) test for preliminary screening of zoo bird sera for antibodies to AIV subtype H5.

引用

页码：882 / 885

页数：4

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