Quorum sensing regulates the transcription of lateral flagellar genes in Vibrio parahaemolyticus

被引：41

作者：

Lu, Renfei ^{[1
,2
]}

Tang, Hao ^{[2
]}

Qiu, Yue ^{[2
]}

Yang, Wenhui ^{[3
]}

Yang, Huiying ^{[3
]}

Zhou, Dongsheng ^{[3
]}

Huang, Xinxiang ^{[2
]}

Hu, Lingfei ^{[3
]}

Zhang, Yiquan ^{[2
]}

机构：

[1] Third Peoples Hosp Nantong, Dept Clin Lab, Nantong 212001, Jiangsu, Peoples R China

[2] Jiangsu Univ, Sch Med, Zhenjiang 212013, Jiangsu, Peoples R China

[3] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

来源：

FUTURE MICROBIOLOGY | 2019年 / 14卷 / 12期

基金：

中国国家自然科学基金;

关键词：

AphA; lateral flagella; OpaR; quorum sensing; Vibrio parahaemolyticus; H-NS; HARVEYI; APHA; EXPRESSION; VIRULENCE; SECRETION; PROTEIN;

D O I：

10.2217/fmb-2019-0048

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: Investigation of the lateral flagellar (Laf) genes transcription by the quorum sensing (QS) regulators AphA and OpaR in Vibrio parahaemolyticus. Materials & methods: Regulation mechanisms were assessed by combined utilization of swarming motility assay, qPCR, LacZ fusion, EMSA and DNase I footprinting. Results: AphA and OpaR oppositely regulate swarming motility and Laf genes. At high cell density, OpaR bound to the regulatory regions of motY-lafK-fliEFGHIJ, fliMNPQR-flhBA, fliDSTKLA-motAB and lafA to repress their transcription. At low cell density, AphA indirectly activated their transcription. Conclusion: OpaR repression of swarming motility was via its direct repression of Laf genes, while AphA exerted its regulatory effect on swarming motility through unknown regulator(s).

引用

页码：1043 / 1053

页数：11

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