Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure

被引：27
作者：
Bisht, Meena [1 ]
Kumar, Awanish [1 ]
Venkatesu, Pannuru [1 ]
机构：
[1] Univ Delhi, Dept Chem, Delhi 110007, India
来源：
PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2016年 / 18卷 / 18期
关键词：
CRYSTALLIZATION ADDITIVES; PROTEIN; RENATURATION; EXTRACTION; STABILITY; SALT;
D O I：
10.1039/c6cp01022a
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The activity of lysozyme over a Micrococcus lysodeikticus cell suspension increased to 13% of the initial value in the presence of 1% v/v ammonium-based ionic liquids after deactivation with 4.0 M urea. This increase in activity reflects the refolding ability of the ionic liquids against the denaturation effects of urea on lysozyme.
引用
收藏
页码：12419 / 12422
页数：4
相关论文
共 24 条
[1]   Influence of ionic liquid and ionic salt on protein against the reactive species generated using dielectric barrier discharge plasma [J].
Attri, Pankaj ;
Sarinont, Thapanut ;
Kim, Minsup ;
Amano, Takaaki ;
Koga, Kazunori ;
Cho, Art E. ;
Choi, Eun Ha ;
Shiratani, Masaharu .
SCIENTIFIC REPORTS, 2015, 5
[2]   Activity and stability of α-chymotrypsin in biocompatible ionic liquids: enzyme refolding by triethyl ammonium acetate [J].
Attri, Pankaj ;
Venkatesu, Pannuru ;
Kumar, Anil .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2011, 13 (07) :2788-2796
[3]   Analysis of the driving force that rule the stability of lysozyme in alkylammonium-based ionic liquids [J].
Bisht, Meena ;
Kumar, Awanish ;
Venkatesu, Pannuru .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 81 :1074-1081
[4]   Unfolding mechanism of lysozyme in various urea solutions: Insights from fluorescence spectroscopy [J].
Chen, Bang ;
Zhang, Hongjia ;
Xi, Wenying ;
Zhao, Liqing ;
Liang, Li ;
Chen, Yantao .
JOURNAL OF MOLECULAR STRUCTURE, 2014, 1076 :524-528
[5]   Partition of proteins with extraction in aqueous two-phase system by hydroxyl ammonium-based ionic liquid [J].
Chen, Jing ;
Wang, Yuzhi ;
Zeng, Qun ;
Ding, Xueqin ;
Huang, Yanhua .
ANALYTICAL METHODS, 2014, 6 (12) :4067-4076
[6]   A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation [J].
Emadi, Saeed ;
Behzadi, Maliheh .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2014, 450 (04) :1339-1344
[7]   Protein solubilising and stabilising ionic liquids [J].
Fujita, K ;
MacFarlane, DR ;
Forsyth, M .
CHEMICAL COMMUNICATIONS, 2005, (38) :4804-4806
[8]   Protic ionic liquids: Properties and applications [J].
Greaves, Tamar L. ;
Drummond, Calum J. .
CHEMICAL REVIEWS, 2008, 108 (01) :206-237
[9]   OPTICAL ACTIVITY OF HUMAN LYSOZYME [J].
HALPER, JP ;
LATOVITZKI, N ;
BERNSTEIN, H ;
BEYCHOK, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (03) :517-+
[10]   Influence of urea and guanidine hydrochloride on lysozyme stability and thermal denaturation; a correlation between activity, protein dynamics and conformational changes [J].
Hedoux, Alain ;
Krenzlin, Stefanie ;
Paccou, Laurent ;
Guinet, Yannick ;
Flament, Marie-Pierre ;
Siepmann, Juergen .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (40) :13189-13196
123