Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species

被引：27

作者：

Goldenberg, O

Herrmann, S

Adam, T

Marjoram, G

Hong, G

Göbel, UB

Graf, B

机构：

[1] Univ Med Berlin, Inst Microbiol & Hyg, Charite, D-10117 Berlin, Germany

[2] Transgenom Ltd, Omaha, NE USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 12期

关键词：

D O I：

10.1128/JCM.43.12.5912-5915.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.

引用

收藏

页码：5912 / 5915

页数：4

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