Secretion of the rotavirus VP8*protein in Lactococcus lactis

被引：10

作者：

Gil, MT

Pérez-Arellano, I

Buesa, J

Pérez-Martínez, G ^{[1
]}

机构：

[1] Inst Agroquim & Technol Alimentos, Dept Biotechnol, Valencia 46100, Spain

[2] Univ Valencia, Hosp Clin Univ, Dept Microbiol, Valencia, Spain

[3] Univ Valencia, Fac Med, Valencia, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 203卷 / 02期

关键词：

rotavirus; heterologous secretion; VP8; Lactococcus;

D O I：

10.1016/S0378-1097(01)00367-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Secretion of the VP8* subunit of the VP4 capsid protein of rotavirus by Lactococcus lactis has been achieved. For this purpose, a secretion vector has been constructed with the lactococcal signal sequence AL9 and the VP8*-encoding gene fragment. The amount of VP8* secreted by L. lactis in the culture supernatant was quantified and visualised by Western blot. Furthermore, it was shown to retain its hemagglutination capability, indicating that the conformation of the secreted peptide may be retaining its biological activity. (C) 2001 Federation of European Microbiological Societies, Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：269 / 274

页数：6

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