Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG-2 hepatocellular carcinoma cells

被引：25

作者：

Liu, Ying ^{[1
]}

Ban, Li-Ying ^{[1
]}

Su, Xi ^{[1
]}

Gao, Shan ^{[1
]}

Liu, Ji-Wei ^{[1
]}

Cui, Xiao-Nan ^{[1
]}

机构：

[1] Dalian Med Univ, Affiliated Hosp 1, Dept Oncol, Dalian 116011, Liaoning, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2015年 / 12卷 / 01期

基金：

中国国家自然科学基金;

关键词：

cinobufacini injection; HepG-2; cells; proliferation; apoptosis; topoisomerase I; topoisomerase II; PROSTATE-CANCER CELLS; DNA; BUFALIN; APOPTOSIS; HUACHANSU; PATHWAYS; THERAPY; DEATH; PHASE;

D O I：

10.3892/mmr.2015.3552

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The present study aimed to investigate the effects of cinobufacini injection on the proliferation and expression of topoisomerases in human HepG-2 hepatocarcinoma cells. The cells were divided into a control group and an experimental group, in which 0.105, 0.21, 0.42 mg/l cinobufacini was injected. Cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide assay, levels of apoptosis were detected using annexin V/propidium iodide staining and cell cycles were analyzed using flow cytometric analysis. The mRNA and protein expression levels of topoisomerase (TOPO) I and TOPO II were determined by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. Cinobufacini injection significantly inhibited the proliferation of the HepG-2 cells (P<0.05), induced apoptosis (P<0.05) in a dose- and time-dependent manner, induced tumor cell arrest at the S phase in a dose-dependent manner, and downregulated the mRNA and protein expression levels of TOPO I and TOPO II (P<0.05) in a dose-dependent manner. Therefore, cinobufacini was found to inhibit human HepG-2 hepatocellular carcinoma cell proliferation, and downregulation of the expression levels of TOPO I and TOPO II may contribute to the effect on proliferation observed in the Hep-G(2) cells following cinobbufacini injection.

引用

页码：1598 / 1604

页数：7

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