Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CN/SDS-PAGE Coupled with Mass Spectrometry

被引：8
作者：
Zhao, Zhiyun
Liu, Hui
Wang, Xinli
Wang, Xiaodong
Li, Zhili [1 ]
机构：
[1] Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biophys & Struct Biol, Beijing 100005, Peoples R China
来源：
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年
基金：
中国国家自然科学基金;
关键词：
HEAT-SHOCK PROTEINS; BLUE-NATIVE ELECTROPHORESIS; GEL-ELECTROPHORESIS; PROTEOMICS; MITOCHONDRIAL; EXPRESSION; TRANSGLUTAMINASE; PATTERNS; VACCINES;
D O I：
10.1155/2011/193052
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Protein complexes are a cornerstone of many biological processes and together they form various types of molecular machinery. A broad understanding of these protein complexes is crucial for revealing and building models of protein function and regulation. Pancreatic cancer is a highly lethal disease which is difficult to diagnose at early stage and even more difficult to cure. In this study, we applied a gradient clear native gel system combined with subsequent second-dimensional SDS-PAGE to separate protein complexes from cell lysates of SW1990 and PANC-1 pancreatic cancer cell lines with different degrees of differentiation. Ten heat-shock-protein-(HSP-) associated protein complexes were separated and identified, and the differentially expressed proteins related to cancers were also found, such as HSP60, protein disulfide-isomerase A4 (ERp72), and transitional endoplasmic reticulum ATPase (TER ATPase).
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页数：8
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