M13 Phage-Functionalized Single-Walled Carbon Nanotubes As Nanoprobes for Second Near-Infrared Window Fluorescence Imaging of Targeted Tumors

被引：231

作者：

Yi, Hyunjung ^{[1
,2
]}

Ghosh, Debadyuti ^{[1
,2
]}

Ham, Moon-Ho ^{[3
]}

Qi, Jifa ^{[1
,2
]}

Barone, Paul W. ^{[3
]}

Strano, Michael S. ^{[3
]}

Belcher, Angela M. ^{[1
,2
,4
]}

机构：

[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[2] MIT, David H Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[4] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

NANO LETTERS | 2012年 / 12卷 / 03期

基金：

美国国家科学基金会;

关键词：

Second near-infrared window fluorescence; single-walled carbon nanotubes; molecular imaging; hybrid imaging probes; nanobiotechnology; IN-VIVO; QUANTUM DOTS; MEMBRANE ANTIGEN; MICE; NANOPARTICLES; PEPTIDES; CANCER; CELLS; MOLECULES; SURGERY;

D O I：

10.1021/nl2031663

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Second near-infrared (NIR) window light (950-1400 nm) is attractive for in vivo fluorescence imaging due to its deep penetration depth in tissues and low tissue autofluorescence. Here we show genetically engineered multifunctional M13 phage can assemble fluorescent single-walled carbon nanotubes (SWNTs) and ligands for targeted fluorescence imaging of tumors. M13-SWNT probe is detectable in deep tissues even at a low dosage of 2 mu g/mL and up to 2.5 cm in tissue-like phantoms. Moreover, targeted probes show specific and up to 4-fold improved uptake in prostate specific membrane antigen positive prostate tumors compared to control nontargeted probes. This M13 phage-based second NIR window fluorescence imaging probe has great potential for specific detection and therapy monitoring of hard-to-detect areas.

引用

页码：1176 / 1183

页数：8

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