Minimizing tip-sample forces in jumping mode atomic force microscopy in liquid

被引：67

作者：

Ortega-Esteban, A. ^{[1
]}

Horcas, I. ^{[2
]}

Hernando-Perez, M. ^{[1
]}

Ares, P. ^{[2
]}

Perez-Berna, A. J. ^{[3
]}

San Martin, C. ^{[3
]}

Carrascosa, J. L. ^{[3
]}

de Pablo, P. J. ^{[1
]}

Gomez-Herrero, J. ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Dept Fis Mat Condensada, Madrid 28049, Spain

[2] Ctr Empresarial Euronova 3, Nanotec Elect SL, Madrid 28760, Spain

[3] Ctr Nacl Biotecnol CNB CSIC, Madrid 28049, Spain

来源：

ULTRAMICROSCOPY | 2012年 / 114卷

关键词：

Atomic force microscopy; Scanning in liquids; Virus; RESOLUTION; MATURATION; VIRUS;

D O I：

10.1016/j.ultramic.2012.01.007

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Control and minimization of tip-sample interaction forces are imperative tasks to maximize the performance of atomic force microscopy. In particular, when imaging soft biological matter in liquids, the cantilever dragging force prevents identification of the tip-sample mechanical contact, resulting in deleterious interaction with the specimen. In this work we present an improved jumping mode procedure that allows detecting the tip-sample contact with high accuracy, thus minimizing the scanning forces (similar to 100 pN) during the approach cycles. To illustrate this method we report images of human adenovirus and T7 bacteriophage particles which are prone to uncontrolled modifications when using conventional jumping mode. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：56 / 61

页数：6

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