Sub-10-nm imaging of nucleic acids using spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON)

被引：2

作者：

Eshein, Adam ^{[1
]}

Li, Yue ^{[2
]}

Dong, Biqin ^{[1
,3
]}

Almassalha, Luay M. ^{[1
]}

Chandler, John E. ^{[1
]}

The-Quyen Nguyen ^{[1
]}

Hujsak, Karl A. ^{[4
]}

Dravid, Vinayak P. ^{[4
]}

Sun, Cheng ^{[3
]}

Zhang, Hao F. ^{[1
]}

Backman, Vadim ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biomed Engn, 2145 Sheridan Rd, Evanston, IL 60208 USA

[2] Northwestern Univ, Appl Phys Program, 2145 Sheridan Rd, Evanston, IL 60208 USA

[3] Northwestern Univ, Dept Mech Engn, 2145 Sheridan Rd, Evanston, IL 60208 USA

[4] Northwestern Univ, Dept Mat Sci & Engn, 2145 Sheridan Rd, Evanston, IL 60208 USA

来源：

OPTICS LETTERS | 2018年 / 43卷 / 23期

基金：

美国国家科学基金会;

关键词：

NUCLEOSOME CORE PARTICLE; FLUORESCENCE; ORGANIZATION; MICROSCOPY; DNA;

D O I：

10.1364/OL.43.005817

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Elucidating chromatin structure in vitro requires resolution below 10 nm to visualize the mononucleosome has been an ongoing challenge. In this work, we achieve sub-10-nm imaging of nucleic acids via spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON) without the use of external labels. SICLON leverages two key innovations: using endogenous nucleotides as the emission source and a custom-made imaging system that can simultaneously record the position and optical spectra of emitting molecules. With a novel spectral regression algorithm that identifies the spectroscopic fingerprints of neighboring molecules that were previously indistinguishable, we demonstrate the utility of SICLON by visualizing unlabeled poly-nucleotides and linear single-stranded DNA fibers with a resolution of 6.2 nm. (c) 2018 Optical Society of America

引用

页码：5817 / 5820

页数：4