RAGE Gene Deletion Inhibits the Development and Progression of Ductal Neoplasia and Prolongs Survival in a Murine Model of Pancreatic Cancer

被引：38

作者：

DiNorcia, Joseph ^{[1
]}

Lee, Minna K. ^{[1
]}

Moroziewicz, Dorota N. ^{[1
]}

Winner, Megan ^{[1
]}

Suman, Paritosh ^{[1
]}

Bao, Fei ^{[2
]}

Remotti, Helen E. ^{[2
]}

Zou, Yu Shan ^{[4
]}

Yan, Shi Fang ^{[4
]}

Qiu, Wanglong ^{[3
]}

Su, Gloria H. ^{[1
,3
]}

Schmidt, Ann Marie ^{[4
]}

Allendorf, John D. ^{[1
]}

机构：

[1] Columbia Univ, Coll Phys & Surg, Dept Surg, New York, NY 10032 USA

[2] Columbia Univ, Coll Phys & Surg, Dept Pathol, New York, NY 10032 USA

[3] Columbia Univ, Coll Phys & Surg, Dept Otolaryngol & Head & Neck Surg, New York, NY 10032 USA

[4] NYU, Dept Med, Langone Med Ctr, New York, NY 10016 USA

来源：

JOURNAL OF GASTROINTESTINAL SURGERY | 2012年 / 16卷 / 01期

关键词：

RAGE; AGER; PanIN; Pancreatic adenocarcinoma; Pancreatic cancer; Pdx1-Cre; Kras; p16; RAGE(-/-) mice; MOUSE MODEL; GROWTH; INFLAMMATION; P16(INK4A); EXPRESSION; CARCINOMA; RECEPTOR; S100P; ADENOCARCINOMA; P19(ARF)-P53;

D O I：

10.1007/s11605-011-1754-9

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The receptor for advanced glycation end-products (RAGE) is implicated in pancreatic tumorigenesis. Activating Kras mutations and p16 inactivation are genetic abnormalities most commonly detected as pancreatic ductal epithelium progresses from intraepithelial neoplasia (PanIN) to adenocarcinoma (PDAC). The aim of this study was to evaluate the effect of RAGE (or AGER) deletion on the development of PanIN and PDAC in conditional Kras (G12D) mice. Pdx1-Cre; LSL-Kras (G12D/+) mice were crossed with RAGE (-/-) mice to generate Pdx1-Cre; LSL-Kras (G12D/+) ; RAGE (-/-) mice. Pdx1-Cre; LSL-Kras (G12D/+); p16 (Ink4a-/-) mice were crossed with RAGE (-/-) mice to generate Pdx1-Cre; LSL-Kras (G12D/+); p16 (Ink4a-/-); RAGE (-/-) mice. Pancreatic ducts were scored and compared to the relevant RAGE (+/+) controls. At 16 weeks of age, Pdx1-Cre; LSL-Kras (G12D/+); RAGE (-/-) mice had significantly fewer high-grade PanIN lesions than Pdx1-Cre; LSL-Kras (G12D/+); RAGE (+/+) controls. At 12 weeks of age, none of the Pdx1-Cre; LSL-Kras (G12D/+); p16 (Ink4a-/-); RAGE (-/-) mice had PDAC compared to a 45.5% incidence of PDAC in Pdx1-Cre; LSL-Kras (G12D/+); p16 (Ink4a-/-); RAGE (+/+) controls. Finally, Pdx1-Cre; LSL-Kras (G12D/+); p16 (Ink4a-/-); RAGE (-/-) mice also displayed markedly longer median survival. Loss of RAGE function inhibited the development of PanIN and progression to PDAC and significantly prolonged survival in these mouse models. Further work is needed to target the ligand-RAGE axis for possible early intervention and prophylaxis in patients at risk for developing pancreatic cancer.

引用

页码：104 / 112

页数：9

共 39 条

[21] RAGE and RAGE ligands in cancer [J].