Validation of a PCR technique for the detection of enteroagreggative Escherichia coli causing traveler's diarrhea

被引：6

作者：

Ruiz-Blázquez, J

Vargas, M

Nataro, JP

Vila, J

Brustenga, JGI

机构：

[1] IDIBAPS, Ctr Salud Int, Hosp Clin, Barcelona, Spain

[2] IDIBAPS, Secc Enfermedades Infecc, Hosp Clin, Barcelona, Spain

[3] IDIBAPS, Microbiol Serv, Hosp Clin, Barcelona, Spain

[4] Univ Maryland, Sch Med, Ctr Vaccine Dev, Dept Pediat Med, Baltimore, MD 21201 USA

[5] Univ Maryland, Sch Med, Ctr Vaccine Dev, Dept Microbiol & Immunol, Baltimore, MD 21201 USA

来源：

ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2005年 / 23卷 / 08期

关键词：

diarrhea; EAEC; HEp-2;

D O I：

10.1157/13078826

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

INTRODUCTION. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS. The gold standard was compared with a PCR method for this purpose. RESULTS. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION. The PCR method studied is a viable alternative for routine detection of EAEC strains.

引用

页码：479 / 481

页数：3

共 10 条

[1] A SENSITIVE AND SPECIFIC DNA PROBE TO IDENTIFY ENTEROAGGREGATIVE ESCHERICHIA-COLI, A RECENTLY DISCOVERED DIARRHEAL PATHOGEN
BAUDRY, B
SAVARINO, SJ
VIAL, P
KAPER, JB
LEVINE, MM
[J]. JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (06) : 1249 - 1251
[2] Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli
Czeczulin, JR
Whittam, TS
Henderson, IR
Navarro-Garcia, F
Nataro, JP
[J]. INFECTION AND IMMUNITY, 1999, 67 (06) : 2692 - 2699
[3] ETIOLOGY AND EPIDEMIOLOGY OF PERSISTENT DIARRHEA IN NORTHEASTERN BRAZIL - A HOSPITAL-BASED, PROSPECTIVE, CASE-CONTROL STUDY
FANG, GD
LIMA, AAM
MARTINS, CV
NATARO, JP
GUERRANT, RL
[J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1995, 21 (02) : 137 - 144
[4] Enteroaggregative Escherichia coli strains as a cause of traveler's diarrhea:: A case-control study
Gascón, J
Vargas, M
Quintó, L
Corachán, M
de Anta, MTJ
Vila, J
[J]. JOURNAL OF INFECTIOUS DISEASES, 1998, 177 (05) : 1409 - 1412
[5] PERSISTENT DIARRHEA IN CHILDREN - EPIDEMIOLOGY, RISK-FACTORS, PATHOPHYSIOLOGY, NUTRITIONAL IMPACT, AND MANAGEMENT
LIMA, AAM
GUERRANT, RL
[J]. EPIDEMIOLOGIC REVIEWS, 1992, 14 : 222 - 242
[6] DETECTION OF AN ADHERENCE FACTOR OF ENTEROPATHOGENIC ESCHERICHIA-COLI WITH A DNA PROBE
NATARO, JP
BALDINI, MM
KAPER, JB
BLACK, RE
BRAVO, N
LEVINE, MM
[J]. JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) : 560 - 565
[7] Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli
Scaletsky, ICA
Fabbricotti, SH
Aranda, KR
Morais, MB
Fagundes-Neto, U
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (04) : 1254 - 1258
[8] DEVELOPMENT OF PCR FOR SCREENING OF ENTEROAGGREGATIVE ESCHERICHIA-COLI
SCHMIDT, H
KNOP, C
FRANKE, S
ALEKSIC, S
HEESEMANN, J
KARCH, H
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) : 701 - 705
[9] Enteropathogens in adult patients with diarrhea and healthy control subjects:: A 1-year prospective study in a Swedish clinic for infectious diseases
Svenungsson, B
Lagergren, Å
Ekwall, E
Evengård, B
Hedlund, KO
Kärnell, A
Löfdahl, S
Svensson, L
Weintraub, A
[J]. CLINICAL INFECTIOUS DISEASES, 2000, 30 (05) : 770 - 778
[10] COMPARISON OF 2 ASSAY-METHODS FOR PATTERNS OF ADHERENCE TO HEP-2 CELLS OF ESCHERICHIA-COLI FROM PATIENTS WITH DIARRHEA
VIAL, PA
MATHEWSON, JJ
DUPONT, HL
GUERS, L
LEVINE, MM
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (05) : 882 - 885

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