Validation of a PCR technique for the detection of enteroagreggative Escherichia coli causing traveler's diarrhea

被引：6

作者：

Ruiz-Blázquez, J

Vargas, M

Nataro, JP

Vila, J

Brustenga, JGI

机构：

[1] IDIBAPS, Ctr Salud Int, Hosp Clin, Barcelona, Spain

[2] IDIBAPS, Secc Enfermedades Infecc, Hosp Clin, Barcelona, Spain

[3] IDIBAPS, Microbiol Serv, Hosp Clin, Barcelona, Spain

[4] Univ Maryland, Sch Med, Ctr Vaccine Dev, Dept Pediat Med, Baltimore, MD 21201 USA

[5] Univ Maryland, Sch Med, Ctr Vaccine Dev, Dept Microbiol & Immunol, Baltimore, MD 21201 USA

来源：

ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2005年 / 23卷 / 08期

关键词：

diarrhea; EAEC; HEp-2;

D O I：

10.1157/13078826

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

INTRODUCTION. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS. The gold standard was compared with a PCR method for this purpose. RESULTS. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION. The PCR method studied is a viable alternative for routine detection of EAEC strains.

引用

收藏

页码：479 / 481

页数：3

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