A fluorescent double stain for visualization of neural tissue by confocal laser scanning microscopy

被引：10

作者：

Kröger, RHH ^{[1
]}

Wagner, HJ ^{[1
]}

机构：

[1] Univ Tubingen, Inst Anat, Abt Zellulare Neurobiol, D-72074 Tubingen, Germany

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1998年 / 84卷 / 1-2期

关键词：

confocal microscopy; three-dimensional; fluorescence; lucifer yellow; DiD;

D O I：

10.1016/S0165-0270(98)00093-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a fast and simple method for a general, fluorescent double stain that differentially labels various cellular components and visualizes all cells in confocal laser scanning microscopy. The technique is useful for two- and three-dimensional visualization of neural tissue and facilitates quantification of a variety of neuroanatomical parameters. Examples from cerebellum and retina are shown to demonstrate the broad applicability. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：87 / 92

页数：6

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