A fluorescent double stain for visualization of neural tissue by confocal laser scanning microscopy

被引：10
|
作者：
Kröger, RHH [1 ]
Wagner, HJ [1 ]
机构：
[1] Univ Tubingen, Inst Anat, Abt Zellulare Neurobiol, D-72074 Tubingen, Germany
来源：
JOURNAL OF NEUROSCIENCE METHODS | 1998年 / 84卷 / 1-2期
关键词：
confocal microscopy; three-dimensional; fluorescence; lucifer yellow; DiD;
D O I：
10.1016/S0165-0270(98)00093-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present a fast and simple method for a general, fluorescent double stain that differentially labels various cellular components and visualizes all cells in confocal laser scanning microscopy. The technique is useful for two- and three-dimensional visualization of neural tissue and facilitates quantification of a variety of neuroanatomical parameters. Examples from cerebellum and retina are shown to demonstrate the broad applicability. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：87 / 92
页数：6
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