Protamines of eutherian species are cysteine-rich molecules that become cross-linked by disulfide bonds during epididymal transit, whereas the protamines of most marsupial species lack cysteine residuals. The present study made use of the differences in protamine structure between eutherian and metatherian mammal spermatozoa to examine the comparative resistance of sperm DNA to oxidative damage in three eutherian species (Mus musculus, Homo sapiens, Sus domesticus) and three metatherian species (Vombatus ursinus, Phascolarctos cinereus, Macropus giganteus). Sperm DNA fragmentation of samples exposed to increasing concentrations of hydrogen peroxide was assessed by means of the two-tailed comet assay. The sperm DNA of the marsupial species studied were significantly more sensitive to oxidative stress than the spermatozoa of eutherian species. Such susceptibility is consistent with the lack of disulfide cross-linking in marsupial sperm chromatin and suggests that the oxidation of thiols to disulfides for chromatin condensation during epididymal transit in eutherian mammals is likely to be important in order to provide stability and protect these cells from the genotoxic effects of adverse environments.
机构:
Institute of Modern Physics, Chinese Academy of Sciences
University of Chinese Academy of SciencesInstitute of Modern Physics, Chinese Academy of Sciences
Fang Liu
Zhuan-Zi Wang
论文数: 0引用数: 0
h-index: 0
机构:
Institute of Modern Physics, Chinese Academy of SciencesInstitute of Modern Physics, Chinese Academy of Sciences
Zhuan-Zi Wang
Wen-Jian Li
论文数: 0引用数: 0
h-index: 0
机构:
Institute of Modern Physics, Chinese Academy of SciencesInstitute of Modern Physics, Chinese Academy of Sciences
Wen-Jian Li
Wei Wei
论文数: 0引用数: 0
h-index: 0
机构:
Institute of Modern Physics, Chinese Academy of SciencesInstitute of Modern Physics, Chinese Academy of Sciences
Wei Wei
Bing-Rong Dang
论文数: 0引用数: 0
h-index: 0
机构:
Institute of Modern Physics, Chinese Academy of SciencesInstitute of Modern Physics, Chinese Academy of Sciences
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
Univ Chinese Acad Sci, Beijing 100049, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
Liu, Fang
Wang, Zhuan-Zi
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
Wang, Zhuan-Zi
Li, Wen-Jian
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
Li, Wen-Jian
Wei, Wei
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
Wei, Wei
Dang, Bing-Rong
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R ChinaChinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China