Biochemical and molecular studies of early blight disease in tomato

被引:19
作者
Patel, Suchita J. [1 ]
Subramanian, R. B. [1 ]
Jha, Yachana S. [1 ]
机构
[1] Sardar Patel Univ, BR Doshi Sch Biosci, Vallabh Vidyanagar 388120, Gujarat, India
关键词
Alternaria solani; Pathogenesis-related enzymes; Simple sequence repeats; Systemic acquired resistance; PATHOGENESIS-RELATED PROTEINS; PHENYLALANINE AMMONIA-LYASE; POLYPHENOL OXIDASE; ALTERNARIA-SOLANI; SALICYLIC-ACID; SSR MARKERS; RESISTANCE; PLANTS; INDUCTION; PEROXIDASE;
D O I
10.1007/s12600-011-0156-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Tomato early blight occurs worldwide and it is prevalent wherever tomatoes are grown. Alternaria solani Sorauer, the causal agent, has been recognized as a serious foliar pathogen of tomato and there are very few cultivars which possess resistance against early blight. Alternaric acid is the major toxin of A. solani. In this study, alternaric acid and fungal culture filtrate were used as an elicitor in NDT-96 (tolerant) and GP-5 (susceptible) tomato varieties in order to study and compare their abilities to induce defense-related enzymes, viz., catalase, peroxidase, beta-1,3 glucanase, phenylalanine-ammonia-lyase (PAL), chitinase and polyphenol-oxidase (PPO) along with total phenols, and total soluble proteins. NDT-96 showed a rapid induction of all these pathogenesis-related enzymes except catalase along with total phenols as compared to GP-5 with both the treatments. Differential expression of total soluble proteins revealed higher protein content in NDT-96 as compared with GP-5. A 49.48 kDa protein was observed to be absent in GP-5. In addition, 25 microsatellite markers (SSR) were screened for polymorphisms among the above mentioned two tomato varieties. Of these, SSR 286 revealed a significant polymorphic band of 108 bp in NDT-96.
引用
收藏
页码:269 / 283
页数:15
相关论文
共 67 条
[1]  
BARBER JM, 1980, J PLANT BREEDING, V97, P135
[2]  
BARKSDALE TH, 1971, PLANT DIS REP, V55, P807
[3]   PEROXIDASE, POLYPHENOLOXIDASE, AND PHENOLS IN RELATION TO RESISTANCE AGAINST PSEUDOMONAS-SYRINGAE PV TOMATO IN TOMATO PLANTS [J].
BASHAN, Y ;
OKON, Y ;
HENIS, Y .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1987, 65 (02) :366-372
[4]   DIFFERENTIAL INDUCTION OF CHALCONE SYNTHASE MESSENGER-RNA ACTIVITY AT THE ONSET OF PHYTOALEXIN ACCUMULATION IN COMPATIBLE AND INCOMPATIBLE PLANT PATHOGEN INTERACTIONS [J].
BELL, JN ;
DIXON, RA ;
BAILEY, JA ;
ROWELL, PM ;
LAMB, CJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (11) :3384-3388
[5]  
BHATIA I S, 1972, Indian Phytopathology, V25, P231
[6]  
BRIAN P. W., 1952, ANN APPL BIOL, V39, P308, DOI 10.1111/j.1744-7348.1952.tb01013.x
[7]   ENHANCED RESISTANCE OF TOMATO PLANTS TO FUSARIUM BY CONTROLLED STIMULATION OF THEIR NATURAL PHENOLIC PRODUCTION [J].
CARRASCO, A ;
BOUDET, AM ;
MARIGO, G .
PHYSIOLOGICAL PLANT PATHOLOGY, 1978, 12 (02) :225-&
[8]   ACTIVE OXYGEN SPECIES IN THE INDUCTION OF PLANT SYSTEMIC ACQUIRED-RESISTANCE BY SALICYLIC-ACID [J].
CHEN, ZX ;
SILVA, H ;
KLESSIG, DF .
SCIENCE, 1993, 262 (5141) :1883-1886
[9]   Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory [J].
Constabel, CP ;
Yip, L ;
Patton, JJ ;
Christopher, ME .
PLANT PHYSIOLOGY, 2000, 124 (01) :285-295
[10]   INDUCTION OF BETA-1,3-GLUCANASE AND CHITINASE ACTIVITY IN COMPATIBLE AND INCOMPATIBLE INTERACTIONS BETWEEN COLLETOTRICHUM-LINDEMUTHIANUM AND BEAN CULTIVARS [J].
DAUGROIS, JH ;
LAFITTE, C ;
BARTHE, JP ;
TOUZE, A .
JOURNAL OF PHYTOPATHOLOGY, 1990, 130 (03) :225-234