The pathways by which the marine diatom Thalassiosira sp. OUC2 biodegrades p-xylene, combined with a mechanistic analysis at the proteomic level

被引：19

作者：

Duan, Weiyan ^{[1
,2
]}

Du, Shuhao ^{[1
,3
]}

Meng, Fanping ^{[1
,3
]}

Peng, Xiaoling ^{[1
,3
]}

Peng, Lihong ^{[1
,3
]}

Lin, Yufei ^{[4
]}

Wang, Guoshan ^{[4
]}

Wu, Jiangyue ^{[4
]}

机构：

[1] Minist Educ, Key Lab Marine Environm & Ecol, Qingdao, Shandong, Peoples R China

[2] Hebei Agr Univ, Ocean Coll, Qinhuangdao, Hebei, Peoples R China

[3] Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Shandong, Peoples R China

[4] Minist Nat Resources Peoples Republ China, Natl Marine Hazard Mitigat Serv, Beijing, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2020年 / 198卷

关键词：

p-Xylene; Thalassiosira sp; Biodegradation; Metabolites; Proteomics; SCENEDESMUS-OBLIQUUS; PHENOLIC-COMPOUNDS; KINETIC-ANALYSIS; TOXICITY; DEGRADATION; WATER; IDENTIFICATION; METABOLISM; BACTERIUM; REMOVAL;

D O I：

10.1016/j.ecoenv.2020.110687

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A marine diatom, Thalassiosira sp. OUC2, was isolated from natural seawater collected from Daya Bay, China. This diatom degraded 1.25-40 mg L-1 p-xylene within five days, at a removal efficiency exceeding 98%. Gas chromatography-mass spectrometer (GC-MS) analysis indicated that p-xylene was converted into 4-methylbenzyl alcohol, p-toluic acid, and p-cresol in the presence of strain OUC2. Meanwhile, proteomic analysis showed that, after exposure to p-xylene, several algal enzymes were significantly upregulated: including monooxygenase, alcohol dehydrogenase, benzaldehyde dehydrogenase, benzoate 1,2-dioxygenase, and catechol 2,3-dioxygenase. Moreover, ecotoxicological tests suggested that the intermediate metabolites were less toxic than the parent compound (p-xylene). Thalassiosira sp. OUC2 may thus be suitable for the remediation of p-xylene-contaminated marine environments.

引用

页数：10

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