Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey

被引:5
作者
Gulacti, Irem [1 ]
Sozdutmaz, Ibrahim [2 ]
Isidan, Hakan [3 ]
机构
[1] Pendik Vet Control & Res Inst, Istanbul, Turkey
[2] Erciyes Univ, Dept Virol, Fac Vet Med, Kayseri, Turkey
[3] Cumhuriyet Univ, Dept Virol, Fac Vet Med, Sivas, Turkey
关键词
Bovine norovirus; molecular epidemiology; Turkey; ENTERIC CALICIVIRUSES; RT-PCR; EPIDEMIOLOGY; GENOTYPE; CATTLE;
D O I
10.3906/vet-1504-67
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase (RdRp) gene. The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR. The nucleotide sequences of partial RdRp fragments from the BNoV isolates including the newly identified Turkish isolates showed more than 86.3% nt and 96.8% aa identity with each other. Interestingly, in a comparison of the nt and aa sequences, one of the Turkish BNoV strains was found to be 100% aa identical with some Italian and Tunisian strains. In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey.
引用
收藏
页码:428 / 433
页数:6
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