Metagenomic analysis reveals gestational diabetes mellitus-related microbial regulators of glucose tolerance

被引:50
作者
Wu, Yanxin [1 ]
Bible, Paul W. [2 ,3 ]
Long, Sizhe [4 ]
Ming, Wai-Kit [1 ]
Ding, Wenjing [1 ]
Long, Yuhang [1 ]
Wen, Xiaofeng [2 ]
Li, Xifang [2 ]
Deng, Xiuli [2 ]
Deng, Yuhua [2 ]
Guo, Shixin [2 ]
Doci, Colleen L. [3 ]
Wei, Lai [2 ]
Chen, Haitian [1 ]
Wang, Zilian [1 ]
机构
[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Guangzhou 510080, Peoples R China
[2] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Peoples R China
[3] Marian Univ, Coll Arts & Sci, Indianapolis, IN USA
[4] Sun Yat Sen Univ, Ctr Informat Technol & Stat, Stat Sect, Affiliated Hosp 1, Guangzhou 510080, Peoples R China
基金
中国国家自然科学基金;
关键词
Gut microbiota; Microbiome; Host-microbiota interaction; Gestational diabetes mellitus; Glucose tolerance; Metagenomic shotgun sequencing; PENTOSE-PHOSPHATE PATHWAY; GUT MICROBIOTA; MATERNAL OBESITY; PREGNANCY; IDENTIFICATION; HYPERGLYCEMIA; ASSOCIATION; EXPOSURE; WEIGHT; HEALTH;
D O I
10.1007/s00592-019-01434-2
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aims Recent studies have suggested a possible association between microbiota and gestational diabetes (GDM). However, the results are inconsistent. Our objective was to investigate further the relationship between GDM and microbiota and verify the potential microbial marker. Methods Two complementary approaches were used for the demonstration. First, we compared the gut microbial composition of 23 GDM patients and 26 non-GDM ethnically Chinese Han pregnant women, by using whole-metagenome shotgun sequencing of their stool samples collected at the third trimester. Second, we used Q-PCR (quantitative polymerase chain reaction) to evaluate the gut microbial composition in the stool samples from another cohort of 150 Chinese pregnant women (113 Control and 37 GDM), to further confirm the potential microbial marker. Results The gut microbiota of GDM women show lower albeit not statistically significant (p = 0.18) alpha diversity at the species level than non-GDM women. However, the species-level beta-diversity or between-sample diversity measured by Bray-Curtis distance shows significant differences (p < 2.2e-16) between the two groups. The species Bacteroides dorei positively correlated with both OGTT (oral glucose tolerance test) 0-Hour (p = 0.0099) and OGTT 1-Hour (p = 0.0070). There is a similar trend between Bacteroides sp. 3_1_33FAA and both OGTT 0-Hour (p = 0.014) and OGTT 1-Hour (p = 0.0101) response variables. The species Alistipes putredinis negatively correlated with OGTT 1-Hour (p = 0.0172) and OGTT 2-Hour (p = 0.0147). Q-PCR validation further confirmed the association between the glucose tolerance loci of Bacteroides dorei and OGTT response. Conclusions Gut microbiome is related to the diabetic status of Chinese women during pregnancy. Specific species such as Bacteroides dorei associate with glucose response and could be potential monitoring and therapeutic microbial markers for GDM.
引用
收藏
页码:569 / 581
页数:13
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