Advances in 3D cell culture for liver preclinical studies

被引：6

作者：

Yao, Ting ^{[1
]}

Zhang, Yi ^{[1
]}

Lv, Mengjiao ^{[1
]}

Zang, Guoqing ^{[1
]}

Ng, Soon Seng ^{[2
]}

Chen, Xiaohua ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Dept Infect Dis, Shanghai 200233, Peoples R China

[2] Imperial Coll London, Fac Med, Dept Metab Digest & Reprod, London W2 1PG, England

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2021年 / 53卷 / 06期

基金：

中国国家自然科学基金;

关键词：

3D cell culture; liver; scaffold; tissue engineering; PRIMARY HUMAN HEPATOCYTES; HEPATIC STEM-CELLS; IN-VITRO; EXTRACELLULAR-MATRIX; TISSUE; DIFFERENTIATION; ORGAN; HEPG2; METABOLISM; TOXICOLOGY;

D O I：

10.1093/abbs/gmab046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 3D cell culture model is an indispensable tool in the study of liver biology in the field of health and disease and the development of clinically relevant products for liver therapies. The 3D culture model captures critical factors of the microenvironmental niche required by hepatocytes for exhibiting optimal phenotypes, thus enabling the pursuit of a range of preclinical studies that are not entirely feasible in conventional 2D cell models. In this review, we highlight the major attributes associated with and the components needed for the development of a functional 3D liver culture model for a range of applications.

引用

页码：643 / 651

页数：9

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