Overexpression of SOX15 inhibits proliferation of NT2/D1 cells derived from a testicular embryonal cell carcinoma

被引：1
作者：
Yan, Hong-Tao [1 ,2 ,4 ,5 ]
Shinka, Toshikatsu [1 ,4 ,5 ]
Sato, Youichi [1 ,4 ,5 ]
Yang, Xin-Jun [1 ,2 ,4 ,5 ]
Chen, Gang [1 ,4 ,5 ]
Sakamoto, Kozue [1 ,4 ,5 ]
Kinoshita, Keigo [1 ,4 ,5 ]
Aburatani, Hiroyuki [3 ]
Nakahori, Yutaka [1 ,4 ,5 ]
机构：
[1] Univ Tokushima, Grad Sch Prote, Fac Med, Dept Human Genet & Publ Hlth, Tokushima 7708503, Japan
[2] Wenzhou Med Coll, Sch Environm Sci & Publ Hlth, Wenzhou 325035, Zhejiang, Peoples R China
[3] Univ Tokyo, Ctr Collaborat Res, Genome Sci Div, Tokyo 1538904, Japan
[4] Core Res Evolut Sci & Technol Corp CREST, Kawaguchi, Saitama 3320012, Japan
[5] Univ Tokushima, 21st Century Ctr Excellence COE Program, Tokushima 7708503, Japan
来源：
MOLECULES AND CELLS | 2007年 / 24卷 / 03期
关键词：
cell cycle; cell proliferation; SOX; SOX15;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.
引用
收藏
页码：323 / 328
页数：6
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