Overexpression of SOX15 inhibits proliferation of NT2/D1 cells derived from a testicular embryonal cell carcinoma

被引：1
作者：
Yan, Hong-Tao [1 ,2 ,4 ,5 ]
Shinka, Toshikatsu [1 ,4 ,5 ]
Sato, Youichi [1 ,4 ,5 ]
Yang, Xin-Jun [1 ,2 ,4 ,5 ]
Chen, Gang [1 ,4 ,5 ]
Sakamoto, Kozue [1 ,4 ,5 ]
Kinoshita, Keigo [1 ,4 ,5 ]
Aburatani, Hiroyuki [3 ]
Nakahori, Yutaka [1 ,4 ,5 ]
机构：
[1] Univ Tokushima, Grad Sch Prote, Fac Med, Dept Human Genet & Publ Hlth, Tokushima 7708503, Japan
[2] Wenzhou Med Coll, Sch Environm Sci & Publ Hlth, Wenzhou 325035, Zhejiang, Peoples R China
[3] Univ Tokyo, Ctr Collaborat Res, Genome Sci Div, Tokyo 1538904, Japan
[4] Core Res Evolut Sci & Technol Corp CREST, Kawaguchi, Saitama 3320012, Japan
[5] Univ Tokushima, 21st Century Ctr Excellence COE Program, Tokushima 7708503, Japan
来源：
MOLECULES AND CELLS | 2007年 / 24卷 / 03期
关键词：
cell cycle; cell proliferation; SOX; SOX15;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.
引用
收藏
页码：323 / 328
页数：6
相关论文
共 34 条
  • [1] Role of Fabp7, a downstream gene of Pax6, in the maintenance of neuroepithelial cells during early embryonic development of the rat cortex
    Arai, Y
    Funatsu, N
    Numayama-Tsuruta, K
    Nomura, T
    Nakamura, S
    Osumi, N
    [J]. JOURNAL OF NEUROSCIENCE, 2005, 25 (42) : 9752 - 9761
  • [2] Id2 reverses cell cycle arrest induced by γ-irradiation in human HaCaT keratinocytes
    Baghdoyan, S
    Lamartine, J
    Castel, D
    Pitaval, A
    Roupioz, Y
    Franco, N
    Duarte, M
    Martin, MT
    Gidrol, X
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (16) : 15836 - 15841
  • [3] Muscle differentiation is antagonized by SOX15, a new member of the SOX protein family
    Béranger, F
    Méjean, C
    Moniot, B
    Berta, P
    Vandromme, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (21) : 16103 - 16109
  • [4] Phylogeny of the SOX family of developmental transcription factors based on sequence and structural indicators
    Bowles, J
    Schepers, G
    Koopman, P
    [J]. DEVELOPMENTAL BIOLOGY, 2000, 227 (02) : 239 - 255
  • [5] Partitioning of IGFBP-5 actions in myogenesis: IGF-independent anti-apoptotic function
    Cobb, LJ
    Salih, DAM
    Gonzalez, I
    Tripathi, G
    Carter, EJ
    Lovett, F
    Holding, C
    Pell, JM
    [J]. JOURNAL OF CELL SCIENCE, 2004, 117 (09) : 1737 - 1746
  • [6] Suppression of growth and tumorigenicity in the prostate tumor cell line M12 by overexpression of the transcription factor SOX9
    Drivdahl, R
    Haugk, KH
    Sprenger, CC
    Nelson, PS
    Tennant, MK
    Plymate, SR
    [J]. ONCOGENE, 2004, 23 (26) : 4584 - 4593
  • [7] SRY, LIKE HMG1, RECOGNIZES SHARP ANGLES IN DNA
    FERRARI, S
    HARLEY, VR
    PONTIGGIA, A
    GOODFELLOW, PN
    LOVELLBADGE, R
    BIANCHI, ME
    [J]. EMBO JOURNAL, 1992, 11 (12) : 4497 - 4506
  • [8] Transcription factor SOX18 is expressed in human coronary atherosclerotic lesions and regulates DNA synthesis and vascular cell growth
    García-Ramírez, M
    Martínez-González, J
    Juan-Babot, JO
    Rodríguez, C
    Badimon, L
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (11) : 2398 - 2403
  • [9] THE HMG DOMAIN OF LYMPHOID ENHANCER FACTOR-I BENDS DNA AND FACILITATES ASSEMBLY OF FUNCTIONAL NUCLEOPROTEIN STRUCTURES
    GIESE, K
    COX, J
    GROSSCHEDL, R
    [J]. CELL, 1992, 69 (01) : 185 - 195
  • [10] Programmed cell death protein 4 suppresses CDK1/cdc2 via induction of p21Waf1/Cip1
    Göke, R
    Barth, P
    Schmidt, A
    Samans, B
    Lankat-Buttgereit, B
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2004, 287 (06): : C1541 - C1546
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