MiniSTR multiplex systems based on non-CODIS loci for analysis of degraded DNA samples

被引：21

作者：

Asamura, R. ^{[1
]}

Fujimori, S. ^{[1
]}

Ota, A. ^{[1
]}

Fukushima, H. ^{[1
]}

机构：

[1] Shinshu Univ, Sch Med, Dept Legal Med, Matsumoto, Nagano 3908621, Japan

来源：

FORENSIC SCIENCE INTERNATIONAL | 2007年 / 173卷 / 01期

关键词：

autosomal miniSTR; quadruplex; non-CODIS loci; Japanese population; degraded DNA;

D O I：

10.1016/j.forsciint.2007.01.015

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

We describe two short amplicon autosomal short tandem repeat (miniSTR) quadruplex systems for eight loci D1S1171, D2S1242, D3S1545, D4S2366, D12S391, D16S3253, D20S161, and D21S1437, unlinked from the combined DNA index system (non-CODIS) loci, using newly designed primer sets. The results of an assay of 411 Japanese individuals showed that polymerase chain reaction (PCR) products within the eight loci were less than 150 bp in size, without the seven additional bases for adenylation. The frequency distributions in the loci showed no deviations from Hardy-Weinberg equilibrium expectations. The accumulated power of discrimination and power of exclusion for the eight loci were 0.9999999991 and 0.998, respectively. For assay of highly degraded DNA, including artificially degraded samples and the degraded forensic casework samples assessed with the present miniSTR quadruplex systems, the systems proved quite effective in analyzing degraded DNA. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：7 / 15

页数：9

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