Ethylene sensitivity and relative air humidity regulate root hydraulic properties in tomato plants

被引:8
作者
Calvo-Polanco, Monica [1 ,2 ]
Ibort, Pablo [1 ]
Molina, Sonia [1 ]
Ruiz-Lozano, Juan Manuel [1 ]
Maria Zamarreno, Angel [3 ]
Maria Garcia-Mina, Jose [3 ]
Aroca, Ricardo [1 ]
机构
[1] Estn Expt Zaidin CSIC, Dept Soil Microbiol & Symbiot Syst, C Prof Albareda 1, Granada 18008, Spain
[2] SupAgro INRA, UMR 5004, Biochim & Physiol Mol Plantes, 2,Pl Viala, F-34060 Montpellier 2, France
[3] Univ Navarra, Dept Environm Biol, Fac Sci, Agr Chem & Biol Grp CMI Roullier, Irunlarrea 1, Pamplona 31008, Spain
关键词
ACC; Aquaporins; Ethylene; Phytohormones; Relative humidity; Root hydraulic conductivity; ARBUSCULAR MYCORRHIZAL SYMBIOSIS; PLASMA-MEMBRANE AQUAPORINS; INDUCED STOMATAL CLOSURE; 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID; WATER TRANSPORT; ABSCISIC-ACID; METHYL JASMONATE; STRESS TOLERANCE; GENE-EXPRESSION; DROUGHT STRESS;
D O I
10.1007/s00425-017-2746-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of ethylene and its precursor ACC on root hydraulic properties, including aquaporin expression and abundance, is modulated by relative air humidity and plant sensitivity to ethylene. Relative air humidity (RH) is a main factor contributing to water balance in plants. Ethylene (ET) is known to be involved in the regulation of root water uptake and stomatal opening although its role on plant water balance under different RH is not very well understood. We studied, at the physiological, hormonal and molecular levels (aquaporins expression, abundance and phosphorylation state), the plant responses to exogenous 1-aminocyclopropane-1-carboxylic acid (ACC; precursor of ET) and 2-aminoisobutyric acid (AIB; inhibitor of ET biosynthesis), after 24 h of application to the roots of tomato wild type (WT) plants and its ET-insensitive never ripe (nr) mutant, at two RH levels: regular (50%) and close to saturation RH. Highest RH induced an increase of root hydraulic conductivity (Lp(o)) of non-treated WT plants, and the opposite effect in nr mutants. The treatment with ACC reduced Lp(o) in WT plants at low RH and in nr plants at high RH. The application of AIB increased Lp(o) only in nr plants at high RH. In untreated plants, the RH treatment changed the abundance and phosphorylation of aquaporins that affected differently both genotypes according to their ET sensitivity. We show that RH is critical in regulating root hydraulic properties, and that Lp(o) is affected by the plant sensitivity to ET, and possibly to ACC, by regulating aquaporins expression and their phosphorylation status. These results incorporate the relationship between RH and ET in the response of Lp(o) to environmental changes.
引用
收藏
页码:987 / 997
页数:11
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