Interaction of Escherichia coli RNase H with modified oligonucleotide duplexes .2. Duplexes formed by oligonucleotides consisting of (1-beta-D-2'-deoxy-threo-pentofuranosyl)thymine residues

被引：0

作者：

Alekseev, YI ^{[1
]}

Gottikh, MB ^{[1
]}

Romanova, EA ^{[1
]}

Shabarova, ZA ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,RUSSIA

来源：

MOLECULAR BIOLOGY | 1996年 / 30卷 / 02期

关键词：

E-coli RNase H; modified oligonucleotide;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A dodecanucleotide consisting of 1-(beta-D-2'-deoxy-threo-pentafuranosyl)thymine residues [d(xT)(12)] was synthesized by the amidophosphite method. Under conditions close to the physiological ones (pH 7.6, 100 mM KCl, 10 mM MgCl2), it formed stable duplexes with rA(12) (T-m = 29 degrees C) and dA(12) (T-m = 30 degrees C). It was shown that d(xT)(12)/rA(12) and d(xT)(12)/dA(12) are not substrates for E. coli RNase H and do not bind to the enzyme.

引用

页码：206 / 208

页数：3