Isolation and characterization of a rice WUSCHEL-type homeobox gene that is specifically expressed in the central cells of a quiescent center in the root apical meristem

被引:214
作者
Kamiya, N
Nagasaki, H
Morikami, A
Sato, Y
Matsuoka, M [1 ]
机构
[1] Nagoya Univ, Biosci Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[2] Chubu Univ, Coll Biosci & Biotechnol, Aichi 4678501, Japan
关键词
homeobox; quiescent center (QC); rice; root apical meristem (RAM); shoot apical meristem (SAM); WUSCHEL;
D O I
10.1046/j.1365-313X.2003.01816.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The Arabidopsis WUSCHEL (WUS) protein, which plays an important role in the specification of the stem cells in the shoot apical meristem (SAM), contains an 'atypical' homeodomain (HD) with extra residues in its loop and turn regions. We speculated that a WUS-type atypical HD protein might also be involved in the specification and maintenance of the root apical meristem (RAM) stem cells of rice. To investigate this possibility, we isolated and characterized a rice WUS -type homeobox gene designated quiescent-center-specific homeobox (QHB ) gene. Using transformants carrying the QHB promoter-GUS and in situ hybridization, we found that QHB was specifically expressed in the central cells of a quiescent center (QC) of the root. During embryogenesis and crown root formation, QHB expression was observed prior to the morphological differentiation of the root. However, we detected different QHB expression patterns in the process of the RAM development, specifically between radicle and crown root formation, suggesting that the cell-fate determination of the QC may be controlled by different mechanisms. We also produced transformants that overexpress QHB or Arabidopsis WUS . These transformants did not form crown roots, but developed multiple shoots from ectopic SAMs with malformed leaves. On the basis of these observations, we propose that the WUS -type homeobox gene is involved in the specification and maintenance of the stem cells (QC cells) in the RAM, by a mechanism similar to that for WUS in the SAM.
引用
收藏
页码:429 / 441
页数:13
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