Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage

被引：25

作者：

Rudi, Knut ^{[1
,2
]}

Hagen, Irina ^{[1
]}

Johnsrud, Bente Carina ^{[1
]}

Skjefstad, Guro ^{[1
]}

Tryland, Ingun ^{[3
]}

机构：

[1] Hedmark Univ Coll, N-2418 Elverum, Norway

[2] NOFIMA, As, Norway

[3] NIVA Norwegian Water Res Inst, Oslo, Norway

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH | 2010年 / 7卷 / 09期

关键词：

viable/dead cells; UV; quantitative PCR; ESCHERICHIA-COLI; PCR; QUANTITY; QUALITY; NUCLEAR; PROBE; WATER;

D O I：

10.3390/ijerph7093376

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.

引用

收藏

页码：3376 / 3381

页数：6

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