Crystallization of piratoxin I, a myotoxic LYS49-phospholipase A2 homologue isolated from the venom of Bothrops pirajai

被引：5
|
作者：
Canduri, F
Mancuso, LC
Soares, AM
Giglio, JR
Ward, RJ
Arni, RK
机构：
[1] UNESP, IBILCE, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
[2] USP, Fac Med Ribeirao Preto, Dept Bioquim, BR-14049900 Ribeirao Preto, SP, Brazil
来源：
TOXICON | 1998年 / 36卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
D O I：
10.1016/S0041-0101(97)00120-7
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Fernanda Canduri, Lit C. Mancuso, Andreimar M. Soares, Jose R. Giglio, Richard J. Ward and Raghuvir K. Arni. Crystallization of piratoxin I, a myotoxic Lys49-phospholipase A(2) homologue isolated from the venom of Bothrops pirajai. Toxicon 36, 547-551, 1998.-Large single crystals of piratoxin I, a Lys49-PLA(2) homologue with low enzymatic activity, have been obtained. The crystals belong to the orthorhombic system space group p2(1)2(1)2(1) and diffract X-raps to a resolution of 2.8 Angstrom. Preliminary analysis reveals the presence of two molecules in the crystallographic asymmetric unit. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：547 / 551
页数：5
相关论文
共 50 条
  • [1] Amino acid sequence of piratoxin-II, a myotoxic Lys49 phospholipase A2 homologue from Bothrops pirajai venom
    Toyama, MH
    Soares, AM
    Wen-Hwa, L
    Polikarpov, I
    Giglio, JR
    Marangoni, S
    BIOCHIMIE, 2000, 82 (03) : 245 - 250
  • [2] Amino acid sequence of a myotoxic Lys49-phospholipase A2 homologue from the venom of Cerrophidion (Bothrops) godmani
    de Sousa, MV
    Morhy, L
    Arni, RK
    Ward, RJ
    Díaz, C
    Gutiérrez, JM
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1998, 1384 (02): : 204 - 208
  • [3] Crystallization and preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with rosmarinic acid
    dos Santos, Juliana I.
    Santos-Filho, Norival A.
    Soares, Andreimar M.
    Fontes, Marcos R. M.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 699 - 701
  • [4] Crystal structure of piratoxin-I:: A calcium-independent, myotoxic phospholipase A2-homologue from Bothrops pirajai venom
    De Azevedo, WF
    Ward, RJ
    Canduri, F
    Soares, A
    Giglio, JR
    Arni, RK
    TOXICON, 1998, 36 (10) : 1395 - 1406
  • [5] Crystal structure of myotoxin II, a monomeric Lys49-Phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani
    Arni, RK
    Fontes, MRM
    Barberato, C
    Gutiérrez, JM
    Díaz, C
    Ward, RJ
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1999, 366 (02) : 177 - 182
  • [6] Crystallization and preliminary X-ray diffraction studies of piratoxin II, a phospholipase A2 isolated from the venom of Bothrops pirajai
    Lee, WH
    Goncalez, MC
    Ramalheira, RMF
    Kuser, PR
    Toyama, MH
    Oliveira, B
    Giglio, JR
    Marangoni, S
    Polikarpov, I
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1998, 54 : 1437 - 1439
  • [7] Inflammasome Activation Induced by a Snake Venom Lys49-Phospholipase A2 Homologue
    Boeno, Charles Nunes
    Paloschi, Mauro Valentino
    Lopes, Jessica Amaral
    Pires, Weverson Luciano
    Setubal, Sulamita da Silva
    Evangelista, Jaina Rodrigues
    Soares, Andreimar Martins
    Zuliani, Juliana Pavan
    TOXINS, 2020, 12 (01)
  • [8] Preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with p-bromophenacyl bromide and α-tocopherol inhibitors
    dos Santos, Juliana I.
    Marchi-Salvador, Daniela P.
    Fernandes, Carlos A. H.
    Silveira, Lucas B.
    Soares, Andreimar M.
    Fontes, Marcos R. M.
    PROTEIN AND PEPTIDE LETTERS, 2007, 14 (07): : 698 - 701
  • [9] Crystallization and preliminary X-ray diffraction studies of piratoxin III, a D-49 phospholipase A2 from the venom of Bothrops pirajai
    Wen-Hwa, L
    Toyama, MH
    Soares, AM
    Giglio, JR
    Marangoni, S
    Polikarpov, I
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 1229 - 1230
  • [10] Structure of a Lys49 phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)
    de Azevedo, WF
    Ward, RJ
    Gutiérrez, JM
    Arni, RK
    TOXICON, 1999, 37 (02) : 371 - 384
12345