Prevalence of drug resistance among HIV-1 treatment-naive patients in Greece during 2003-2015: Transmitted drug resistance is due to onward transmissions

被引：0

作者：

Paraskevis, D. ^{[1
]}

Kostaki, E. ^{[1
]}

Magiorkinis, G. ^{[1
]}

Gargalianos, P. ^{[2
]}

Xylomenos, G. ^{[2
]}

Magiorkinis, E. ^{[1
]}

Lazanas, M. ^{[3
]}

Chini, M. ^{[3
]}

Nikolopoulos, G. ^{[4
]}

Skoutelis, A. ^{[5
]}

Papastamopoulos, V. ^{[5
]}

Antoniadou, A. ^{[6
]}

Papadopoulos, A. ^{[6
]}

Psichogiou, M. ^{[7
]}

Daikos, G. L. ^{[7
]}

Oikonomopoulou, M. ^{[1
]}

Zavitsanou, A. ^{[1
]}

Chrysos, G. ^{[8
]}

Paparizos, V. ^{[9
]}

Kourkounti, S. ^{[9
]}

Sambatakou, H. ^{[10
]}

Sipsas, N. V. ^{[11
]}

Lada, M. ^{[12
]}

Panagopoulos, P. ^{[13
]}

Maltezos, E. ^{[13
]}

Drimis, S. ^{[8
]}

Hatzakis, A. ^{[1
]}

机构：

[1] Univ Athens, Dept Hyg Epidemiol & Med Stat, Athens, Greece

[2] G Genimatas GH, Dept Internal Med 1, Athens, Greece

[3] Red Cross Hosp, Internal Med Dept Infect Dis 3, Athens, Greece

[4] Univ Cyprus, Med Sch, Nicosia, Cyprus

[5] Evaggelismos GH, Dept Med & Infect Dis 5, Athens, Greece

[6] Univ Athens, Med Sch, Attikon GH, Dept Med 4, Athens, Greece

[7] Univ Athens, Med Sch, Attikon GH, Dept Med 1, Athens, Greece

[8] Tzaneio GH, Dept Internal Med, Piraeus, Greece

[9] A Syngros Hosp Dermatol & Venereol, HIV AIDS Unit, Athens, Greece

[10] Univ Athens, Med Sch, Hippokrat GH, HIV Unit,Dept Internal Med 2, Athens, Greece

[11] Univ Athens, Med Sch, Laikon GH, Dept Pathophysiol 1, Athens, Greece

[12] Sismanogle GH, Dept Internal Med 2, Athens, Greece

[13] Democritus Univ Thrace, Univ GH, Dept Internal Med, Alexandroupolis, Greece

来源：

INFECTION GENETICS AND EVOLUTION | 2017年 / 54卷

基金：

英国惠康基金;

关键词：

HIV-1; Resistance; Local transmission networks; NNRTIs; Phylogenetic analysis; Onward transmissions; MOLECULAR EPIDEMIOLOGY; 1ST-LINE REGIMENS; NORTHERN GREECE; INDIVIDUALS; MUTATIONS; SEX; MEN; INFECTION; CLUSTERS; COHORT;

D O I：

10.1016/j.meegid.2017.07.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: The prevalence of HIV-1 drug resistance among treatment-naive patients ranges between 8.3% and 15% in Europe and North America. Previous studies showed that subtypes A and B were the most prevalent in the Greek HIV-1 epidemic. Our aim was to estimate the prevalence of resistance among drug naive patients in Greece and to investigate the levels of transmission networking among those carrying resistant strains. Methods: HIV-1 sequences were determined from 3428 drug naive HIV-1 patients, in Greece sampled during 01/01/2003-30/6/2015. Transmission clusters were estimated by means of phylogenetic analysis including as references sequences from patients failing antiretroviral treatment in Greece and sequences sampled globally. Results: The proportion of sequences with SDRMs was 5.98% (n = 205). The most prevalent SDRMs were found for NNRTIs (3.76%), followed by N(t) RTIs (2.28%) and PIs (1.02%). The resistance prevalence was 22.2% based on all mutations associated with resistance estimated using the HIVdb resistance interpretation algorithm. Resistance to NNRTIs was the most common ( 16.9%) followed by PIs (4.9%) and N(t) RTIs (2.8%). The most frequently observed NNRTI resistant mutations were E138A (7.7%), E138Q (4.0%), K103N (2.3%) and V179D (1.3%). The majority of subtype A sequences (89.7%; 245 out of 273) with the dominant NNRTI resistance mutations (E138A, K103N, E138Q, V179D) were found to belong to monophyletic clusters suggesting regional dispersal. For subtype B, 68.1% (139 out of 204) of resistant strains (E138A, K103N, E138Q V179D) belonged to clusters. For N(t)RTI-resistance, evidence for regional dispersal was found for 27.3% and 21.6% of subtype A and B sequences, respectively. Conclusions: The TDR rate based on the prevalence of SDRM is lower than the average rate in Europe. However, the prevalence of NNRTI resistance estimated using the HIVdb approach, is high in Greece and it is mostly due to onward transmissions among drug-naive patients. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：183 / 191

页数：9

共 42 条

[1] Lack of evidence for the selection of E138 mutations by first-generation non-nucleoside reverse transcriptase inhibitors in patients infected with HIV-1 non-B subtypes [J].

Abecasis, Ana B. ;

van Laethem, Kristel ;

Theys, Kristof .

AIDS, 2015, 29 (08) :987-988

[2] Analysis of HIV-1 pol sequences from Panama: Identification of phylogenetic clusters within subtype B and detection of antiretroviral drug resistance mutations [J].

Ahumada-Ruiz, Sara ;

Flores-Figueroa, Dario ;

Toala-Gonzalez, Ivan ;

Thomson, Michael M. .

INFECTION GENETICS AND EVOLUTION, 2009, 9 (05) :933-940

[3]

[Anonymous], EURO SURVEILL

[4]

Antoniadou ZA, 2014, AIDS RES HUM RETROV, V30, P225, DOI [10.1089/aid.2013.0166, 10.1089/AID.2013.0166]

[5] Drug Resistance Mutations for Surveillance of Transmitted HIV-1 Drug-Resistance: 2009 Update [J].

Bennett, Diane E. ;

Camacho, Ricardo J. ;

Otelea, Dan ;

Kuritzkes, Daniel R. ;

Fleury, Herve ;

Kiuchi, Mark ;

Heneine, Walid ;

Kantor, Rami ;

Jordan, Michael R. ;

Schapiro, Jonathan M. ;

Vandamme, Anne-Mieke ;

Sandstrom, Paul ;

Boucher, Charles A. B. ;

van de Vijver, David ;

Rhee, Soo-Yon ;

Liu, Tommy F. ;

Pillay, Deenan ;

Shafer, Robert W. .

PLOS ONE, 2009, 4 (03)

[6] Transmission networks of drug resistance acquired in primary/early stage HIV infection [J].

Brenner, Bluma G. ;

Roger, Micho ;

Moisi, Danma D. ;

Oliveira, Maureen ;

Hardy, Isabelle ;

Turgel, Reuven ;

Charest, Hugues ;

Routy, Jean-Pierre ;

Wainberg, Mark A. .

AIDS, 2008, 22 (18) :2509-2515

[7] Systematic review to determine the prevalence of transmitted drug resistance mutations to rilpivirine in HIV-infected treatment-naive persons [J].

Calvez, Vincent ;

Marcelin, Anne-Genevieve ;

Vingerhoets, Johan ;

Hill, Andrew ;

Hadacek, Blanca ;

Moecklinghoff, Christiane .

ANTIVIRAL THERAPY, 2016, 21 (05) :405-412

[8] Transmitted Drug Resistance and Phylogenetic Relationships Among Acute and Early HIV-1-Infected Individuals in New York City [J].

Castor, Delivette ;

Low, Andrea ;

Evering, Teresa ;

Karmon, Sharon ;

Davis, Brandi ;

Figueroa, Amir ;

LaMar, Melissa ;

Garmon, Donald ;

Mehandru, Saurabh ;

Markowitz, Martin .

JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2012, 61 (01) :1-8

[9]

DHHS Panel on Antiretroviral Guidelines for Adults and Adolescents, GUIDELINES USE ANTIR

[10] Detection and characterization of porcine caliciviruses in Italy [J].

Di Bartolo, Ilaria ;

Tofani, Silvia ;

Angeloni, Giorgia ;

Ponterio, Eleonora ;

Ostanello, Fabio ;

Ruggeri, Franco Maria .

ARCHIVES OF VIROLOGY, 2014, 159 (09) :2479-2484

← 1 2 3 4 5 →