Survey of aflatoxins in rice from Iran using immunoaffinity column clean-up and HPLC with fluorescence detection

被引：20
|
作者：
Feizy, J. [1 ]
Beheshti, H. R. [1 ]
Fahim, N. Khoshbakht [1 ]
Janati, S. S. Fakoor [1 ]
Davari, G. [1 ]
机构：
[1] Testa Qual Control Lab, Mashhad, Iran
来源：
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE | 2010年 / 3卷 / 04期
关键词：
chromatography; HPLC; quality assurance; risk assessment; toxicology; aflatoxins; rice; OCHRATOXIN-A; LIVER-CANCER; DIETARY AFLATOXINS; PARBOILED RICE; MYCOTOXINS; CONTAMINATION; PRODUCTS; EXPOSURE; PEANUTS; FOODS;
D O I：
10.1080/19440049.2010.516456
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A study was undertaken to determine levels of aflatoxins in rice. A total of 261 rice samples were analyzed by HPLC using a method was based on the extraction of 50 g of finely ground rice plus 5 g NaCl with 200 ml of 80% methanol. After filtration and immunoaffinity clean-up, 20 mu l was injected onto the HPLC. HPLC analysis was carried out using a Genesis RP C18 column (250 x 4.6, 4 mu m I.D.) and a mobile phase with a linear gradient of water/methanol/acetonitrile (6 : 2 : 2 v/v) over 16 min. Aflatoxins were determined after post-column derivatisation with iodine by fluorescence detection at excitation and emission wavelengths of 365 and 445 nm, respectively. It was found that 68.9% of the rice samples contained aflatoxin B1 at levels greater than 0.2 ng g-1.
引用
收藏
页码：263 / 267
页数：5
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