Survey of aflatoxins in rice from Iran using immunoaffinity column clean-up and HPLC with fluorescence detection

被引：20

作者：

Feizy, J. ^{[1
]}

Beheshti, H. R. ^{[1
]}

Fahim, N. Khoshbakht ^{[1
]}

Janati, S. S. Fakoor ^{[1
]}

Davari, G. ^{[1
]}

机构：

[1] Testa Qual Control Lab, Mashhad, Iran

来源：

FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE | 2010年 / 3卷 / 04期

关键词：

chromatography; HPLC; quality assurance; risk assessment; toxicology; aflatoxins; rice; OCHRATOXIN-A; LIVER-CANCER; DIETARY AFLATOXINS; PARBOILED RICE; MYCOTOXINS; CONTAMINATION; PRODUCTS; EXPOSURE; PEANUTS; FOODS;

D O I：

10.1080/19440049.2010.516456

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A study was undertaken to determine levels of aflatoxins in rice. A total of 261 rice samples were analyzed by HPLC using a method was based on the extraction of 50 g of finely ground rice plus 5 g NaCl with 200 ml of 80% methanol. After filtration and immunoaffinity clean-up, 20 mu l was injected onto the HPLC. HPLC analysis was carried out using a Genesis RP C18 column (250 x 4.6, 4 mu m I.D.) and a mobile phase with a linear gradient of water/methanol/acetonitrile (6 : 2 : 2 v/v) over 16 min. Aflatoxins were determined after post-column derivatisation with iodine by fluorescence detection at excitation and emission wavelengths of 365 and 445 nm, respectively. It was found that 68.9% of the rice samples contained aflatoxin B1 at levels greater than 0.2 ng g-1.

引用

页码：263 / 267

页数：5

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